Applied Biosystems 7900HT Fast Real-Time PCR System

    Equipment/facility: Equipment

      Description

      The acknowledged gold standard in real-time PCR

      Uses
      Gene expression quantification
      SNP determination
      Copy number variation

      Specifications
      384-well peltier-based block
      Calibrated for 4 dyes
      Inclusion of ROX in the master mix normalizes wells to each other
      10-20 µl volume for standard gene expression assays
      As little as 5 µl can be used for SNP determination assays, saving on cost
      Low-density array cards can also be used

      Details
      Applied Biosystems Twister Autoloader allows multiple plates to be loaded automatically.
      Reserve system for use on the Core Cal via dnaLIMS.

      Related services
      RNA and DNA samples can also be quantified by the Facility, using Taqman assays from nucleic acid by absolute or relative quantification.

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      quantitative polymerase chain reaction
      assays
      nucleic acids
      gold
      gene expression
      DNA
      sampling