Beckman Coulter FC500

    Equipment/facility: Service


      A dual-laser flow cytometer with detection of up to 5 colors of fluorescence; users run samples with minimal help after initial training

      Particle size sensitivity and range: Resolves 0.5um particles from background with a maximum detection of up to 40um diameter particle.
      Lasers: 2 (488nm, 633nm)
      Simultaneous colors: 5
      Instrument can accept samples from: 12mm x 75mm tubes only (provided by MCF)
      Instrument can accept tubes manually OR automatically run 32 in a row
      Researchers can use independently following 2-hour hands-on training session
      BSL-1 materials only (must be fixed if BSL-2 or greater)
      Software: CXP software controls the instrument.
      Listmode data files can be collected and reanalyzed in offline flow cytometry software packages such as FCS Express or FlowJo.


      Flow cytometry
      Software packages