Gene-targeted (knockout) mouse production

    Equipment/facility: Service

      Description

      Production of targeted mutant mice by embryonic stem cell microinjection
      What you will need
      Investigators should have:

      A PSU IACUC approved protocol so that chimeric progeny can be transferred to your protocols at weaning
      Consulted with the Animal Resources Program to acquire mouse space in one of the PSU campus facilities
      Evidence of a screening assay (either PCR or Southern), for your mutant allele, so you can detect germline transmission
      Investigators who want to supply their own targeted embryonic stem cell line must have commercial documentation that the cell line was MAP tested for murine adventitious agents prior to expansion

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      Gene Knockout Techniques
      Knockout Mice
      Animal Care Committees
      Cell Line
      Embryonic Stem Cells
      Documentation
      Alleles
      Polymerase Chain Reaction
      Mouse Embryonic Stem Cells