The Penn State Genomics Core Facility at University Park has recently upgraded its capabilities with the acquisition of a PacBio Sequel DNA sequencer.
The Sequel uses Single Molecule Real Time (SMRT) technology to produce long reads, uniform coverage, and high consensus accuracy. The Sequel’s 10-20 kb reads are ideal for de novo sequencing of genomes, full length transcript sequencing, and sequencing of long amplicons, and its SMRT sequencing can also be used for epigenetic analysis by directly detecting DNA base modifications using polymerase kinetics.
The capabilities of the Sequel will complement the short read sequencing applications currently performed on the Facility’s Illumina HiSeq 2500 and Illumina MiSeq.
The Genomics Core Facility at University Park provides services using several different next-generation sequencing platforms. Applications supported include:
Whole genome and transcriptome sequencing of non-model organisms
Amplicon sequencing for metagenomic studies
Differential expression analysis of mRNA and miRNA
ChIP and RIP sequencing