Genomics Core Facility

    Equipment/facility: Facility

    • Location

      Genomics Core Facility Huck Institutes of the Life Sciences Penn State University 407 Chandlee Building University Park, PA 16802

      United States

    Description

    Providing next-generation sequencing and a number of traditional genomic services to the Penn State community as well as those outside the University

    The Penn State Genomics Core Facility at University Park has recently upgraded its capabilities with the acquisition of a PacBio Sequel DNA sequencer.

    The Sequel uses Single Molecule Real Time (SMRT) technology to produce long reads, uniform coverage, and high consensus accuracy. The Sequel’s 10-20 kb reads are ideal for de novo sequencing of genomes, full length transcript sequencing, and sequencing of long amplicons, and its SMRT sequencing can also be used for epigenetic analysis by directly detecting DNA base modifications using polymerase kinetics.

    The capabilities of the Sequel will complement the short read sequencing applications currently performed on the Facility’s Illumina HiSeq 2500 and Illumina MiSeq.

    The Genomics Core Facility at University Park provides services using several different next-generation sequencing platforms. Applications supported include:
    Whole genome and transcriptome sequencing of non-model organisms
    Amplicon sequencing for metagenomic studies
    Differential expression analysis of mRNA and miRNA
    Degradome sequencing
    ChIP and RIP sequencing

    Fingerprint

    DNA
    Genes
    Molecules
    Kinetics
    Genomics
    Messenger RNA