Illumina HiSeq 2500

    Equipment/facility: Equipment

      Description

      This next-generation sequencing instrument produces medium-length reads and can be used for a variety of applications.

      Specifications
      2 flow chips
      8 lane high-output mode and 2 lane rapid-run mode available
      Up to 1.5 billion single reads or 3 billion paired reads per high-output flow chip
      Up to 300 million single reads or 600 million paired reads per rapid-run flow chip
      Fragment sequencing up to 100 nt in high-output and 150 nt in rapid-run
      Paired end sequencing up to 100 x 100 nt in high-output and 150 x 150 in rapid-run
      Sample multiplexing available

      Details
      This next-generation sequencing instrument produces medium-length reads and can be used for a variety of applications, including (but not limited to):



      ChIP-Seq
      RIP-Seq
      RNA-Seq
      miRNA/small RNA
      Degradome sequencing
      Whole genome resequencing
      De novo sequencing
      Utilizing barcoding, up to 96 samples per lane can be analyzed simultaneously.

      Data is produced in the FASTQ format.

      This instrument is used by Genomics Core Facility staff in conjunction with provided next-generation sequencing services.

      Fingerprint

      sequencing
      output
      genome
      multiplexing
      format