Illumina MiSeq

    Equipment/facility: Equipment

      Description

      This instrument is essentially a small version of the HiSeq 2500.

      Specifications
      15 million single reads or 30 million paired reads per run
      Fragment sequencing up to 250 nt
      Paired end sequencing up to 250 x 250 nt
      Sample multiplexing available

      Details
      The sequencing chemistry is similar to the HiSeq, and all libraries compatible with the HiSeq can be sequenced with the MiSeq.

      The MiSeq produces a modest number of reads; therefore, it is best used for pilot projects or for applications requiring small numbers of reads such as de novo sequencing or resequencing of small genomes, amplicon sequencing, and miRNA/small RNA sequencing.

      Sample multiplexing is available.

      Data is produced in the FASTQ format.

      This instrument is used by Genomics Core Facility staff in conjunction with provided next-generation sequencing services.

      Fingerprint

      RNA Sequence Analysis
      Genomics
      MicroRNAs
      Libraries
      Genome