Jansi UV microscope

    Equipment/facility: Equipment

      Description

      Tryptophan fluorescence at 360 nm, following deep UV excitation at 280 nm, is the basis for this rapid and noninvasive imaging microscope for distinguishing protein and salt crystals in situ.

      Uses
      Distinguishing protein and salt crystals in situ

      Specifications
      Users can choose to scan plates in Brightfield or UV modes alone, or both modes together.
      Excitation is from the top - in the episcopic mode - and, therefore, the microscope is usable with both sitting and hanging drops.
      JANSi UVEX microscope has been built with a very sensitive camera (40% absolute quantum efficiency at 360 nm, as compared to 20 - 30% efficiency normally found in other cameras) to improve detection efficiency, and highly transmissive (~90% transmittance) filters and other optical elements to improve collection efficiency.
      The optical system and the camera are highly efficient, giving images with good contrast even with low-intensity deep-UV excitation.
      Light sources for excitation and brightfield illumination are compact LED array modules. LED sources are quiet, easier to operate and do not generate any IR radiation that can heat the sample.

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      microscopes
      cameras
      excitation
      light emitting diodes
      salts
      proteins
      tryptophan
      crystals
      quantum efficiency
      transmittance
      light sources
      modules
      illumination
      filters
      heat
      fluorescence
      radiation