Kintek SX 300 Stopped Flow Spectrometer (SEESL - SFS)

    Equipment/facility: Equipment

    • Location

      University Park, PA 16802

      United States

    Description

    This facility houses a Kintek SX 300 stopped flow spectrometer (SFS) state-of-the art instrument equipped with an excitation light source coupled with a monochromator, two emission channels for fluorescence (photomultipliers) and an absorbance photodiode. A shutter as well as an ability to interface light into reactions has been custom designed for this instrument. Applications for this customizable SFS include:

    Enzyme kinetics
    Nanoparticle catalyst based kinetic measurements
    Fluorescence anisotropy
    Simple chemical kinetics
    Liposome and polymersome based membrane protein transport measurments (water and ions)
    Light driven ion transport measurements in rhodopsins
    Servo-Motor Drive

    The SF-300X stopped-flow has a powerful, computer-controlled servo motor drive that provides precise and reproducible mixing. Double mixing experiments can be performed easily while consuming minimal volumes of precious samples and affording a miniml fast reaction time of only 5 milliseconds. The brushless high performance motor is digitaly controlled with a high torque, low inertia, digital power supply to essentially provide instantaneous starts and stops of the drive syringes.

    Fluoresence - Absorbance Measurements

    The SF-300 stopped-flow uses interchangeable observation cells. A choice of four observation cells is available, and each can record either absorbance or fluorescence or both simultaneously.

    Sub-Millisecond Dead-Time

    The instrument has the capability to produce a dead time of 0.85 milliseconds

    Simple Sample-Load Volume

    A unique two-position valve is used to allow easy and efficient sample loading. In one position, the three syringes are connected to their individual sample load ports. In the other position, the three syringes can be driven to force the mixing of reactants into the observation cell. A 20 microliter minimum sample volume is required to perform the analysis.

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    Syringes
    Spectrometers
    Fluorescence
    Ions
    Membrane Transport Proteins
    Monochromators
    Photomultipliers
    Photodiodes
    Light sources
    Torque
    Catalysts
    Kinetics
    Water
    Enzymes
    Experiments