Nikon N-SIM/N-STORM

    Equipment/facility: Equipment

      Description

      This microscope consolidates two super resolution techniques on one microscope stand.
      N-SIM (structured illumination microscopy)

      Double the Resolution of Conventional Optical Microscopes (xy 115 nm, z 300 nm)
      Applicable to any sample prepared for conventional fluorescence microscopy – no special fluorophore needed
      Fast Temporal Resolution of Approx. 1 sec/frame
      Specifications
      Motorized inverted Nikon ECLIPSE Ti-E TIRF microscope with perfect focus system
      Lasers: 405 nm, 488 nm, 561 nm, 640 nm
      Objective lens:
      CFI SR Apochromat TIRF 100×oil (NA1.49)
      CFI 20x
      ORCA-Flash 4.0 sCMOS camera
      NIS-Elements Ar
      For more information visit https://www.nikoninstruments.com/Products/Super-resolution/N-SIM-E-Super-Resolution


      N-STORM (STochastic Optical Reconstruction Microscopy)

      The N-STORM super-resolution microscope provides 10 times that of conventional optical microscopes
      Lateral (XY) resolution ~20-30nm
      Axial (Z) resolution ~50nm
      For more information visit https://www.nikoninstruments.com/Products/Super-resolution/N-STORM-Super-Resolution

      Fig. 1. Mitochondria in FluoCells® Prepared Slide #1 bovine pulmonary artery endothelial cells (BPAEC) imaged with wide-field (left) and SIM super resolution (right). It is clearly shown that one can obtain images at a higher resolution with SIM. Ex: 561 nm; Label: MitoTracker® Red CMXRos; Microscope: Nikon N-SIM/N-STORM Super Resolution (newly installed). Bar=500 nm.

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      SIM
      microscopes
      microscopy
      mitochondria
      products
      arteries
      optical microscopes
      temporal resolution
      chutes
      flash
      illumination
      lenses