Thermo LTQ Orbitrap Velos ETD

    Equipment/facility: Equipment

      Description

      A hybrid mass spectrometer which incorporates a linear ion trap and a high-resolution Orbitrap mass analyzers

      Uses
      The linear ion trap permits selection and fragmentation of precursor ions of interest, and the Orbitrap performs Fourier-transform high-resolution analyses of the precursor and fragment ions.
      Fragmentation capabilities include collisional ion activation (CID and HCD) and electron-based ion activation, electron-transfer dissociation (ETD).
      The HCD cell in the LTQ facilitates quantitation of isobarically-labeled peptides for differential protein expression analysis or other applications requiring tandem mass tags (e.g. TMT or iTRAQ).
      The ETD option is used for phosphopeptide analysis.
      Peptide fragments generated by ETD provide complementary information to the conventional CID-generated information which is critical in post-translational modification (PTM) analysis and de novo sequencing.
      The Dionex UltiMate 3000 nano-LC system can operate in both 1D and 2D LC mode, where the 2D LC mode facilitates separation of complex mixtures through two orthogonal separation approaches: reverse phase and strong cation exchange.

      Fingerprint

      Ions
      Electrons
      Chemical activation
      Phosphopeptides
      Peptide Fragments
      Mass spectrometers
      Complex Mixtures
      Cations
      Ion exchange
      Fourier transforms
      Peptides
      Proteins