Transgenic mouse production

    Equipment/facility: Service

      Description

      Production of transgenic mice by pronuclear injection
      What you will need
      Investigators should have:

      A PSU IACUC approved protocol so that any transgenic progeny can be transferred to your protocols at weaning
      Evidence of a screening assay, either PCR or Southern, for your proposed transgene
      Consulted with the Animal Resources Program to acquire mouse space in one of the PSU campus facilities
      Consulted with us to establish protocols for isolation of and preparation of DNA constructs

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      Animal Care Committees
      Transgenic Mice
      Polymerase Chain Reaction
      DNA