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Research Output 1975 2019

1990
158 Citations (Scopus)

The function of ras genes in: Saccharomyces cerevisiae

Broach, J. & Deschenes, R. J., Jan 1 1990, In : Advances in Cancer Research. 54, C, p. 79-139 61 p.

Research output: Contribution to journalArticle

ras Genes
Saccharomyces cerevisiae
Proteins
Adenylyl Cyclases
Yeasts
1991
208 Citations (Scopus)

Cloning Genes by Complementation in Yeast

Rose, M. D. & Broach, J., Jan 1 1991, In : Methods in Enzymology. 194, C, p. 195-230 36 p.

Research output: Contribution to journalArticle

Cloning
Yeast
Organism Cloning
Genes
Yeasts
131 Citations (Scopus)

Evidence suggesting that the ARS elements associated with silencers of the yeast mating-type locus HML do not function as chromosomal DNA replication origins

Dubey, D. D., Davis, L. R., Greenfeder, S. A., Ong, L. Y., Zhu, J., Broach, J. R., Newlon, C. S. & Huberman, J. A., Jan 1 1991, In : Molecular and cellular biology. 11, 10, p. 5346-5355 10 p.

Research output: Contribution to journalArticle

Replication Origin
Telomere
DNA Replication
Yeasts
Plasmids
66 Citations (Scopus)

Mutations in the HML E silencer of Saccharomyces cerevisiae yield metastable inheritance of transcriptional repression

Mahoney, D. J., Marquardt, R., Shei, G. J., Rose, A. B. & Broach, J., Jan 1 1991, In : Genes and Development. 5, 4, p. 605-615 11 p.

Research output: Contribution to journalArticle

Saccharomyces cerevisiae
Mutation
Genes
DNA-Binding Proteins
DNA Replication
163 Citations (Scopus)

RAS genes in Saccharomyces cerevisiae: signal transduction in search of a pathway

Broach, J. R., Jan 1991, In : Trends in Genetics. 7, 1, p. 28-33 6 p.

Research output: Contribution to journalArticle

ras Proteins
Saccharomycetales
Saccharomyces cerevisiae
Signal Transduction
Cell Cycle
39 Citations (Scopus)

Ras-regulated signaling processes in Saccharomyces cerevisiae

Broach, J., Jan 1 1991, In : Current Opinion in Genetics and Development. 1, 3, p. 370-377 8 p.

Research output: Contribution to journalArticle

ras Proteins
Guanine Nucleotides
GTP Phosphohydrolases
Post Translational Protein Processing
Saccharomyces cerevisiae
129 Citations (Scopus)

The CDC25 Protein of Saccharomyces cerevisiae Promotes Exchange of Guanine Nucleotides Bound to Ras

Jones, S., Vignais, M. L. & Broach, J., Jan 1 1991, In : Molecular and Cellular Biology. 11, 5, p. 2641-2646 6 p.

Research output: Contribution to journalArticle

ras-GRF1
Saccharomyces cerevisiae Proteins
Guanine Nucleotides
Galactosidases
Guanosine Triphosphate
101 Citations (Scopus)

The Saccharomyces cerevisiae YAK1 gene encodes a protein kinase that is induced by arrest early in the cell cycle

Garrett, S., Menold, M. M. & Broach, J. R., Aug 1991, In : Molecular and cellular biology. 11, 8, p. 4045-4052 8 p.

Research output: Contribution to journalArticle

Protein Kinases
Saccharomyces cerevisiae
Cell Cycle
Adenosine Monophosphate
Genes
1992
7 Citations (Scopus)

Direct simulation of yeast 2-μm circle plasmid amplification

Russo, F. D., Scherson, I. & Broach, J., Apr 7 1992, In : Journal of Theoretical Biology. 155, 3, p. 369-385 17 p.

Research output: Contribution to journalArticle

Yeast
Amplification
plasmids
Circle
Plasmids
28 Citations (Scopus)

Donor locus selection during Saccharomyces cerevisiae mating type interconversion responds to distant regulatory signals

Weiler, K. S. & Broach, J., 1992, In : Genetics. 132, 4, p. 929-942 14 p.

Research output: Contribution to journalArticle

Donor Selection
Saccharomyces cerevisiae
Chromosomes
Chromosome Structures
Chromatin
138 Citations (Scopus)

Inactivation of the protein phosphatase 2A regulatory subunit a results in morphological and transcriptional defects in Saccharomyces cerevisiae

Van Zyl, W., Huang, W., Sneddon, A. A., Stark, M., Camier, S., Werner, M., Marck, C., Sentenac, A. & Broach, J. R., Nov 1992, In : Molecular and cellular biology. 12, 11, p. 4946-4959 14 p.

Research output: Contribution to journalArticle

Protein Phosphatase 2
Saccharomyces cerevisiae
RNA Polymerase III
Transfer RNA
Phenotype
20 Citations (Scopus)

The 45- and 104-kDa forms of phosphatidate phosphatase from Saccharomyces cerevisiae are regulated differentially by phosphorylation via cAMP-dependent protein kinase

Quinlan, J. J., Nickels, J. T., Wu, W. I., Lin, Y. P., Broach, J. & Carman, G. M., Jan 1 1992, In : Journal of Biological Chemistry. 267, 25, p. 18013-18020 8 p.

Research output: Contribution to journalArticle

Phosphatidate Phosphatase
Phosphorylation
Cyclic AMP-Dependent Protein Kinases
Yeast
Saccharomyces cerevisiae
1993
678 Citations (Scopus)

Transcriptional silencing in yeast is associated with reduced nucleosome acetylation

Braunstein, M., Rose, A. B., Holmes, S. G., David Allis, C. & Broach, J., Jan 1 1993, In : Genes and Development. 7, 4, p. 592-604 13 p.

Research output: Contribution to journalArticle

Nucleosomes
Acetylation
Yeasts
Telomere
Histones
1994
60 Citations (Scopus)
ras-GRF1
Guanine Nucleotide Exchange Factors
ras Proteins
Nucleotides
Yeast
50 Citations (Scopus)

TPD1 of Saccharomyces cerevisiae encodes a protein phosphatase 2C-like activity implicated in tRNA splicing and cell separation

Robinson, M. K., Van Zyl, W. H., Phizicky, E. M. & Broach, J. R., Jun 1994, In : Molecular and cellular biology. 14, 6, p. 3634-3645 12 p.

Research output: Contribution to journalArticle

Cell Separation
Transfer RNA
Saccharomyces cerevisiae
RNA Precursors
Genes
1995
26 Citations (Scopus)

Mutations affecting donor preference during mating type interconversion in Saccharomyces cerevisiae

Weiler, K. S., Szeto, L. & Broach, J., Jan 1 1995, In : Genetics. 139, 4, p. 1495-1510 16 p.

Research output: Contribution to journalArticle

Saccharomyces cerevisiae
Donor Selection
Alleles
Mutation
Chromosome Segregation
92 Citations (Scopus)

Nutrient availability and the RAS/cyclic AMP pathway both induce expression of ribosomal protein genes in Saccharomyces cerevisiae but by different mechanisms

Neuman-Silberberg, F. S., Bhattacharya, S. & Broach, J. R., Jan 1 1995, In : Molecular and cellular biology. 15, 6, p. 3187-3196 10 p.

Research output: Contribution to journalArticle

Ribosomal Proteins
Cyclic AMP
Saccharomyces cerevisiae
Food
Genes
10 Citations (Scopus)

Oncogenes and cell proliferation. Editorial overview

Levine, A. J. & Broach, J., Jan 1 1995, In : Current Opinion in Genetics and Development. 5, 1, p. 1-4 4 p.

Research output: Contribution to journalArticle

Oncogenes
Cell Proliferation
67 Citations (Scopus)

Ras membrane targeting is essential for glucose signaling but not for viability in yeast

Bhattacharya, S., Chen, L., Broach, J. R. & Powers, S., Mar 28 1995, In : Proceedings of the National Academy of Sciences of the United States of America. 92, 7, p. 2984-2988 5 p.

Research output: Contribution to journalArticle

ras Proteins
Yeasts
Cell Membrane
Glucose
Membranes
55 Citations (Scopus)

Yeast silencers can act as orientation-dependent gene inactivation centers that respond to environmental signals

Shei, G. J. & Broach, J. R., Jul 1995, In : Molecular and cellular biology. 15, 7, p. 3496-3506 11 p.

Research output: Contribution to journalArticle

Gene Silencing
Yeasts
Transcriptional Silencer Elements
Glucose
Replication Origin
1996
168 Citations (Scopus)

A ceramide-activated protein phosphatase mediates ceramide-induced G1 arrest of Saccharomyces cerevisiae

Nickels, J. T. & Broach, J. R., Feb 15 1996, In : Genes and Development. 10, 4, p. 382-394 13 p.

Research output: Contribution to journalArticle

Ceramides
Phosphoprotein Phosphatases
Saccharomyces cerevisiae
Yeasts
Signal Transduction
316 Citations (Scopus)

Efficient transcriptional silencing in Saccharomyces cerevisiae requires a heterochromatin histone acetylation pattern

Braunstein, M., Sobel, R. E., Allis, C. D., Turner, B. M. & Broach, J. R., Jan 1 1996, In : Molecular and cellular biology. 16, 8, p. 4349-4356 8 p.

Research output: Contribution to journalArticle

Heterochromatin
Acetylation
Histones
Saccharomyces cerevisiae
Lysine
194 Citations (Scopus)

High-throughput screening for drug discovery

Broach, J. R. & Thorner, J., Nov 7 1996, In : Nature. 384, 6604 SUPPL., p. 14-16 3 p.

Research output: Contribution to journalReview article

Drug Discovery
Pharmaceutical Preparations
Proteins
67 Citations (Scopus)

Silencers are required for inheritance of the repressed state in yeast

Holmes, S. G. & Broach, J., Apr 15 1996, In : Genes and Development. 10, 8, p. 1021-1032 12 p.

Research output: Contribution to journalArticle

Yeasts
Maintenance
Saccharomyces
Heterochromatin
Chromatin
48 Citations (Scopus)

Yeast α mating factor structure-activity relationship derived from genetically selected peptide agonists and antagonists of Ste2p

Manfredi, J. P., Klein, C., Herrero, J. J., Byrd, D. R., Trueheart, J., Wiesler, W. T., Fowlkes, D. M. & Broach, J. R., Aug 27 1996, In : Molecular and cellular biology. 16, 9, p. 4700-4709 10 p.

Research output: Contribution to journalArticle

Mating Factor
Haploidy
Structure-Activity Relationship
Saccharomyces cerevisiae
Yeasts
1997
68 Citations (Scopus)

DNA in transcriptionally silent chromatin assumes a distinct topology that is sensitive to cell cycle progression

Bi, X. & Broach, J., Jan 1 1997, In : Molecular and Cellular Biology. 17, 12, p. 7077-7087 11 p.

Research output: Contribution to journalArticle

Chromatin
Cell Cycle
DNA
Nucleosomes
Heterochromatin
65 Citations (Scopus)

Hyperactivation of the silencing proteins, Sir2p and Sir3p, causes chromosome loss

Holmes, S. G., Rose, A. B., Steuerle, K., Saez, E., Sayegh, S., Lee, Y. M. & Broach, J., Mar 1 1997, In : Genetics. 145, 3, p. 605-614 10 p.

Research output: Contribution to journalArticle

Poisons
Chromosomes
Histones
Chromosomal Instability
Chromatin
13 Citations (Scopus)

Oncogenes and cell proliferation.

Broach, J. & Levine, A. J., Jan 1 1997, In : Current opinion in genetics & development. 7, 1, p. 1-6 6 p.

Research output: Contribution to journalEditorial

Oncogenes
Cell Division
Cell Cycle
Cell Proliferation
19 Citations (Scopus)

Role of 1/4 protein in donor locus selection during mating type interconversion

Szeto, L. & Broach, J., Feb 1 1997, In : Molecular and cellular biology. 17, 2, p. 751-759 9 p.

Research output: Contribution to journalArticle

Donor Selection
Alleles
Genes
Homeodomain Proteins
Proteins

Web alert. Oncogenes and cell proliferation

Broach, J. & Levine, A. J., Jan 1 1997, In : Current Opinion in Genetics and Development. 7, 1, 1 p.

Research output: Contribution to journalReview article

Oncogenes
Internet
Cell Proliferation
Developmental Biology
Search Engine
38 Citations (Scopus)

α2p controls donor preference during mating type interconversion in yeast by inactivating a recombinational enhancer of chromosome III

Szeto, L., Fafalios, M. K., Zhong, H., Vershon, A. K. & Broach, J., Aug 1 1997, In : Genes and Development. 11, 15, p. 1899-1911 13 p.

Research output: Contribution to journalArticle

Donor Selection
Chromosomes
Yeasts
Repressor Proteins
Untranslated RNA
1998
4 Citations (Scopus)

Cloning and characterization of a mouse homologue of xenopus PSP24 LPA receptor

Kawasawa, Y., Kume, K. & Shimizu, T., Dec 1 1998, In : FASEB Journal. 12, 8, p. A1458

Research output: Contribution to journalArticle

Lysophosphatidic Acid Receptors
Cloning
Xenopus
Organism Cloning
Oocytes
75 Citations (Scopus)

Efficient transition to growth on fermentable carbon sources in Saccharomyces cerevisiae requires signaling through the Ras pathway

Jiang, Y., Davis, C. & Broach, J. R., Dec 1 1998, In : EMBO Journal. 17, 23, p. 6942-6951 10 p.

Research output: Contribution to journalArticle

Yeast
Saccharomyces cerevisiae
Carbon
Cyclic AMP-Dependent Protein Kinases
Growth
126 Citations (Scopus)

Identification of surrogate agonists for the human FPRL-1 receptor by autocrine selection in yeast

Klein, C., Paul, J. I., Sauvé, K., Schmidt, M. M., Arcangeli, L., Ransom, J., Trueheart, J., Manfredi, J. P., Broach, J. R. & Murphy, A. J., Dec 1 1998, In : Nature Biotechnology. 16, 13, p. 1334-1337 4 p.

Research output: Contribution to journalArticle

Formyl Peptide Receptor
Yeast
Peptides
G-Protein-Coupled Receptors
Yeasts
129 Citations (Scopus)

New assay technologies for high-throughput screening

Silverman, L., Campbell, R. & Broach, J., Jan 1 1998, In : Current Opinion in Chemical Biology. 2, 3, p. 397-403 7 p.

Research output: Contribution to journalArticle

High-Throughput Screening Assays
Fluorescence Spectrometry
Assays
Screening
Throughput

New technologies and modern approaches to drug discovery

Broach, J., Dec 1 1998, In : FASEB Journal. 12, 8

Research output: Contribution to journalArticle

Drug Discovery
Lead compounds
chemistry
Robotics
Oligonucleotide Array Sequence Analysis
36 Citations (Scopus)

p53 mutations isolated in yeast based on loss of transcription factor activity: Similarities and differences from p53 mutations detected in human tumors

Epstein, C. B., Attiyeh, E. F., Hobson, D. A., Silver, A. L., Broach, J. & Levine, A. J., Apr 23 1998, In : Oncogene. 16, 16, p. 2115-2122 8 p.

Research output: Contribution to journalArticle

Transcription Factors
Yeasts
Mutation
Neoplasms
Alleles
221 Citations (Scopus)

Sphingoid base 1-phosphate phosphatase: A key regulator of sphingolipid metabolism and stress response

Mandala, S. M., Thornton, R., Tu, Z., Kurtz, M. B., Nickels, J., Broach, J., Menzeleev, R. & Spiegel, S., Jan 6 1998, In : Proceedings of the National Academy of Sciences of the United States of America. 95, 1, p. 150-155 6 p.

Research output: Contribution to journalArticle

Sphingolipids
Ceramides
Phosphoric Monoester Hydrolases
Yeasts
Phosphates
1999
45 Citations (Scopus)

Immunological analysis of yeast chromatin

Meluh, P. B. & Broach, J., Jan 1 1999, In : Methods in enzymology. 304, p. 414-430 17 p.

Research output: Contribution to journalArticle

Yeast
Chromatin
56 Citations (Scopus)

The yeast HML I silencer defines a heterochromatin domain boundary by directional establishment of silencing

Bi, X., Braunstein, M., Shei, G. J. & Broach, J. R., Oct 12 1999, In : Proceedings of the National Academy of Sciences of the United States of America. 96, 21, p. 11934-11939 6 p.

Research output: Contribution to journalArticle

Heterochromatin
Chromatin
Yeasts
Chromosomes
Insulator Elements
254 Citations (Scopus)

Tor proteins and protein phosphatase 2A reciprocally regulate Tap42 in controlling cell growth in yeast

Jiang, Y. & Broach, J. R., May 17 1999, In : EMBO Journal. 18, 10, p. 2782-2792 11 p.

Research output: Contribution to journalArticle

Protein Phosphatase 2
Cell growth
Sirolimus
Yeast
Phosphorylation
112 Citations (Scopus)

UAS(rpg) can function as a heterochromatin boundary element in yeast

Bi, X. & Broach, J. R., May 1 1999, In : Genes and Development. 13, 9, p. 1089-1101 13 p.

Research output: Contribution to journalArticle

Insulator Elements
Heterochromatin
Ribosomes
Yeasts
Genes
2000
16 Citations (Scopus)

Brain-specific expression of novel G-protein-coupled receptors, with homologies to Xenopus PSP24 and human GPR45

Imamura, Y., Kume, K., Nakade, S., Haga, H., Izumi, T. & Shimizu, T., Oct 5 2000, In : Biochemical and Biophysical Research Communications. 276, 3, p. 952-956 5 p.

Research output: Contribution to journalArticle

G-Protein-Coupled Receptors
Xenopus
Brain
Clone Cells
Complementary DNA
138 Citations (Scopus)

Carboxyl methylation of the phosphoprotein phosphatase 2A catalytic subunit promotes its functional association with regulatory subunits in vivo

Wu, J., Tolstykh, T., Lee, J., Boyd, K., Stock, J. B. & Broach, J., Nov 1 2000, In : EMBO Journal. 19, 21, p. 5672-5681 10 p.

Research output: Contribution to journalArticle

Methylation
Phosphoprotein Phosphatases
Methyltransferases
Catalytic Domain
Yeast

Expression of functional cxcr4 in saccharomyces cells: a model system for analysis of receptor interactions

Zhang, W., Wang, Z., Manfredi, J. F. J., Fujii, N., Pei, G., Broach, J. & Peiper, S. C., Dec 1 2000, In : Blood. 96, 11 PART I

Research output: Contribution to journalArticle

Saccharomyces
Systems Analysis
CXCR4 Receptors
Signal transduction
Protein Sorting Signals
16 Citations (Scopus)

Mammalian PSP24s (α and β isoforms) are not responsive to lysophosphatidic acid in mammalian expression systems

Imamura, Y., Kume, K., Izumi, T. & Shimizu, T., Oct 5 2000, In : Biochemical and Biophysical Research Communications. 276, 3, p. 957-964 8 p.

Research output: Contribution to journalArticle

Lysophosphatidic Acid Receptors
Assays
Protein Isoforms
Xenopus
Serum Response Element
2001
26 Citations (Scopus)

CAC3 (MSI1) suppression of RAS2G19V is independent of chromatin assembly factor I and mediated by NPR1

Johnston, S. D., Enomoto, S., Schneper, L., McClellan, M. C., Twu, F., Montgomery, N. D., Haney, S. A., Broach, J. R. & Berman, J., Feb 26 2001, In : Molecular and cellular biology. 21, 5, p. 1784-1794 11 p.

Research output: Contribution to journalArticle

Chromatin Assembly and Disassembly
Cyclic AMP
Ubiquitin
Chromatin
Signal Transduction
51 Citations (Scopus)

Chromosomal boundaries in S. cerevisiae

Bi, X. & Broach, J., Apr 1 2001, In : Current Opinion in Genetics and Development. 11, 2, p. 199-204 6 p.

Research output: Contribution to journalReview article

Insulator Elements
Chromatin
Saccharomyces cerevisiae
Heterochromatin
Nucleosomes
6 Citations (Scopus)

Genetic selection in Saccharomyces of mutant mammalian adenylyl cyclases with elevated basal activities

Haney, S. A., Xu, J., Lee, S. Y., Ma, C. L., Duzic, E., Broach, J. & Manfredi, J. P., Aug 14 2001, In : Molecular Genetics and Genomics. 265, 6, p. 1120-1128 9 p.

Research output: Contribution to journalArticle

Saccharomyces
Genetic Selection
Adenylyl Cyclases
Genetic Suppression
HEK293 Cells
8 Citations (Scopus)

Sensitivity of wild type and mutant ras alleles to Ras specific exchange factors: Identification of factor specific requirements

Nielsen, K. H., Gredsted, L., Broach, J. & Willumsen, B. M., Apr 19 2001, In : Oncogene. 20, 17, p. 2091-2100 10 p.

Research output: Contribution to journalArticle

ras Proteins
Alleles
ras Genes
GTP Phosphohydrolases
Transcriptional Activation