Bruce Stanley, PhD

    • 1845 Citations
    • 26 h-Index
    1982 …2019

    Research output per year

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    Personal profile

    Research interests

    The primary current focus of Dr. Bruce Stanley's lab is development of mass spectrometric and bioinformatic methods for quantitative proteomic analysis and biomarker discovery.

    These methods include 1D and 2D liquid chromatographic separations of both whole proteins and proteolytically digested peptide fragments; the use of iTRAQ isotope-coded affinity tags; the development of improved mass spec analysis software (with commercial and other academic centers); the development of improved MALDI plate surfaces and MALDI matrix deposition methods (in conjunction with the Penn State Nanofabrications Facility) and improved bioinformatic and biostatistical methods (e.g., for calculating the false discovery rates required in large scale proteomic studies).

    Past goals of the lab research program have been to understand the mechanisms of control of intracellular levels of rapidly degraded proteins and how this subclass of proteins is involved in control of cell growth and differentiation, particularly how enzyme half-life changes contribute to rapid cell proliferation. The enzymes of the polyamine biosynthetic pathway, particularly S-adenosylmethionine decarboxylase, have been studied both as general model proteins to define proteolytic signals which target proteins for rapid proteolysis, and for their specific contributions to control of cell growth. Experimental approaches include cell culture and transfection studies, in vitro expression of wildtype versus mutant proteins, and the development of new in vitro systems to study protein degradation. Structure-function studies are done by specific replacement of amino acid residues in the model proteins, and assessment of the effect of these mutations on either degradative rate or other enzymologic characteristics of the proteins.

    Finally, the lab maintains an interest in arginine/nitric oxide/ornithine metabolism and growth control, particularly how changes in substrate levels may act as control points in metabolic flux independently of changes in enzyme activity.

    Education/Academic qualification

    Postdoctoral Fellowship, Penn State College of Medicine

    … → 1989

    PhD, Cornell University

    … → 1987

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    Projects

    Instrumentation for Confocal Imaging and Analysis

    Stanley, B.

    7/1/026/30/03

    Project: Research project

    Instrumentation for Flow Cytometry/Cell Sorting

    Stanley, B.

    4/1/023/31/03

    Project: Research project

    INSTRUMENTATION OF PEPTIDE ANALYSIS

    Stanley, B.

    4/1/013/31/02

    Project: Research project

    Research Output

    Omega-3 Fatty Acids Responsive Proteins and Reduction in Breast Density in Obese Postmenopausal Women

    Sun, Y. W., Xu, H., Benitez, G., Chen, K. M., Stanley, A., Stanley, B., Zhu, J., Thompson, H., Manni, A. & El-Bayoumy, K., Sep 6 2019, In : Journal of Proteome Research. 18, 9, p. 3461-3469 9 p.

    Research output: Contribution to journalArticle

  • Proteomic analysis of labial saliva of the generalist cabbage looper (Trichoplusia ni) and its role in interactions with host plants

    Rivera-Vega, L. J., Stanley, B. A., Stanley, A. & Felton, G. W., May 1 2018, In : Journal of Insect Physiology. 107, p. 97-103 7 p.

    Research output: Contribution to journalArticle

  • 5 Scopus citations

    Cacao residues in vessels from Chocolá, an early Maya polity in the southern Guatemalan piedmont, determined by semi-quantitative testing and high-performance liquid chromatography

    Kaplan, J., Paredes Umaña, F., Hurst, W. J., Sun, D., Stanley, B., Barba Pingarrón, L. & Obregon Cardona, M., Jun 1 2017, In : Journal of Archaeological Science: Reports. 13, p. 526-534 9 p.

    Research output: Contribution to journalArticle

  • 2 Scopus citations

    Fall armyworm-associated gut bacteria modulate plant defense responses

    Acevedo, F. E., Peiffer, M., Tan, C. W., Stanley, B. A., Stanley, A., Wang, J., Jones, A. G., Hoover, K., Rosa, C., Luthe, D. & Felton, G., Feb 2017, In : Molecular Plant-Microbe Interactions. 30, 2, p. 127-137 11 p.

    Research output: Contribution to journalArticle

  • 27 Scopus citations

    Quantitative proteomic analysis of the fall armyworm saliva

    Acevedo, F. E., Stanley, B. A., Stanley, A., Peiffer, M., Luthe, D. S. & Felton, G. W., Jul 2017, In : Insect Biochemistry and Molecular Biology. 86, p. 81-92 12 p.

    Research output: Contribution to journalArticle

  • 12 Scopus citations