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Research interests

Dr. Clare Sample’s lab focuses on the Epstein-Barr virus, one of the seven human viruses associated with cancer. EBV-associated cancers are largely restricted to B-cell or epithelial cancers. Her research is divided between the latent lifecycle of the virus in B cells, which is associated with cellular proliferation, and the lifecycle in stratified epithelial cells, where the default is high levels of viral replication in the differentiated layers.

In B cells, EBV drives cellular proliferation to enlarge the pool of infected cells, eventually establishing long-term latency in memory B cells. Dr. Sample has investigated the function of the latency-associated EBNA3A and EBNA3C that are essential for EBV-driven proliferation and demonstrated that these are transcription factors that function through a cellular intermediate, the CSL protein, which is the intracellular signaling protein of the Notch pathway.

More recently, her lab has demonstrated that the EBNA3 proteins inhibit the cellular pathways that mediate G1 arrest. The lifecycle of EBV in epithelial compartment is less well-understood despite the fact that EBV must cross the epithelial barrier to infect a new host and that the most common EBV-associated cancers are epithelial-derived. In 2014, Dr. Sample’s lab established the use of organotypic cultures to study the lifecycle of EBV in epithelial cells in stratified epithelium. Unlike epithelial cells in monolayer culture, EBV can infect stratified epithelium, where differentiation spontaneously induces productive replication (new virus production). Her team has recently identified a viral protein that plays an important role in viral spread and may also be involved in viral entry.

Fingerprint Dive into the research topics where Clare Sample is active. These topic labels come from the works of this person. Together they form a unique fingerprint.

  • 1 Similar Profiles
Human Herpesvirus 4 Medicine & Life Sciences
Human herpesvirus 4 Agriculture & Biology
Epstein-Barr Virus Nuclear Antigens Medicine & Life Sciences
nuclear antigens Agriculture & Biology
Proteins Medicine & Life Sciences
B-Lymphocytes Medicine & Life Sciences
Burkitt Lymphoma Medicine & Life Sciences
Nuclear Proteins Medicine & Life Sciences

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Projects 1988 2021

Epstein-Barr Virus Infections
Human Herpesvirus 4
Epithelium
Infection
Viruses
Epstein-Barr Virus Infections
Human Herpesvirus 4
Epithelium
Epithelial Cells
Viral Proteins
Human Herpesvirus 4
Proteins
B-Lymphocytes
National Institute of Allergy and Infectious Diseases (U.S.)
Transplantation
Human Herpesvirus 4
B-Lymphocytes
Viral Proteins
Neoplasms
Proteins

Research Output 1985 2017

  • 2034 Citations
  • 20 h-Index
  • 31 Article
  • 3 Chapter
  • 1 Review article
1 Citation (Scopus)

Generation and infection of organotypic cultures with Epstein-Barr virus

Temple, R. M., Meyers, C. & Sample, C., Jan 1 2017, Methods in Molecular Biology. Humana Press Inc., p. 65-78 14 p. (Methods in Molecular Biology; vol. 1532).

Research output: Chapter in Book/Report/Conference proceedingChapter

Human Herpesvirus 4
Epstein-Barr Virus Infections
Epithelial Cells
Infection
Life Cycle Stages
62 Citations (Scopus)
Human Herpesvirus 4
Epithelium
Epithelial Cells
Herpesviridae
Infection
9 Citations (Scopus)

Epstein-Barr Virus Nuclear Antigen 3A Promotes Cellular Proliferation by Repression of the Cyclin-Dependent Kinase Inhibitor p21WAF1/CIP1

Tursiella, M. L., Bowman, E. R., Wanzeck, K. C., Throm, R. E., Liao, J., Zhu, J. & Sample, C. E., Oct 1 2014, In : PLoS pathogens. 10, 10

Research output: Contribution to journalArticle

Epstein-Barr Virus Nuclear Antigens
Cyclin-Dependent Kinases
Tumor Suppressor Protein p14ARF
Cell Proliferation
Cell Line
3 Citations (Scopus)

Vorinostat downregulates CD30 and decreases brentuximab vedotin efficacy in human lymphocytes

Hasanali, Z. S., Epner, E. M., Feith, D. J., Loughran, T. P. & Sample, C. E., Dec 1 2014, In : Molecular cancer therapeutics. 13, 12, p. 2784-2792 9 p.

Research output: Contribution to journalArticle

Down-Regulation
Lymphocytes
Therapeutics
Neoplasms
vorinostat
19 Citations (Scopus)

Contributions of CTCF and DNA methyltransferases DNMT1 and DNMT3B to Epstein-Barr virus restricted latency

Hughes, D. J., Marendy, E. M., Dickerson, C. A., Yetming, K. D., Sample, C. E. & Sample, J. T., Jan 1 2012, In : Journal of virology. 86, 2, p. 1034-1045 12 p.

Research output: Contribution to journalArticle

Virus Latency
Human herpesvirus 4
methyltransferases
Methyltransferases
Human Herpesvirus 4