Huacheng Luo, PhD

    • 421 Citations
    • 4 h-Index
    20092019
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    Personal profile

    Research interests

    Dr. Huacheng Luo’s current studies are focused on cancer/tumor genetics and epigenetics research fields. One of his current projects involves targeting CTCF boundary remodels chromatin domain and reprograms HOX gene transcription in acute myeloid leukemia. In this research, he employed a pooled CRISPR-Cas9 genetic knockout (KO) library screening to interrogate CTCF binding motifs in all HOX loci and identified a critical CTCF boundary (CBS7/9) located at the edge of TAD encompassing the posterior HOXA genes. He found that CBS7/9 chromatin boundary is critical for initiating and maintaining aberrant expression of posterior HOXA genes (HOXA9-HOXA13).

    To further investigate the role of CBS7/9 chromatin boundary in HOX gene organization and regulation, he then carried out genome wide ChIP-seq, ATAC-seq, 4C-seq and RNA-seq analysis to examine the global changes in chromatin domain organization and corresponding gene expression patterns between control and CBS7/9 knockout AML cells. Depletion of the CBS7/9 boundary function leads to expansion of repressive chromatin structure into posterior HOXA domain, blocking enhancer/promoter chromatin accessibility and their associated regulatory networks, decreases in HOXA9 associated oncogenic transcription program and eventually prolonged survival of transplanted AML mouse models. The CTCF boundaries in the oncogene loci such as CBS7/9 may serve as novel therapeutic targets for the treatment of myeloid malignancies.

    Dr. Luo is also focused on the studies of the HOX loc lncRNAs function in human normal and malignant hematopoiesis, including HOTTIP and HoxBlinc lncRNAs. He found that HOTTIP/HoxBlinc lncRNA preferentially recognizes and binds to noncoding regulatory regions in the AML genome and specifically regulates genes important for hematopoietic and myeloid lineage differentiation.

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    • 18 Similar Profiles
    Chromatin Medicine & Life Sciences
    Epithelial-Mesenchymal Transition Medicine & Life Sciences
    Clustered Regularly Interspaced Short Palindromic Repeats Medicine & Life Sciences
    Neoplasms Medicine & Life Sciences
    Genes Chemical Compounds
    Stem Cells Medicine & Life Sciences
    Gene expression Chemical Compounds
    Histone Demethylases Medicine & Life Sciences

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    Research Output 2009 2019

    • 421 Citations
    • 4 h-Index
    • 6 Article
    • 3 Chapter

    HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

    Luo, H., Sobh, A., Vulpe, C. D., Brewer, E., Dovat, S., Qiu, Y. & Huang, S., Mar 31 2019, In : Journal of visualized experiments : JoVE. 145

    Research output: Contribution to journalArticle

    Clustered Regularly Interspaced Short Palindromic Repeats
    Chromatin
    Screening
    Genes
    Gene Expression
    2 Citations (Scopus)

    CTCF boundary remodels chromatin domain and drives aberrant HOX gene transcription in acute myeloid leukemia

    Luo, H., Wang, F., Zha, J., Li, H., Yan, B., Du, Q., Yang, F., Sobh, A., Vulpe, C., Drusbosky, L., Cogle, C., Chepelev, I., Xu, B., Nimer, S. D., Licht, J., Qiu, Y., Chen, B., Xu, M. & Huang, S., Aug 23 2018, In : Blood. 132, 8, p. 837-848 12 p.

    Research output: Contribution to journalArticle

    Transcription
    Acute Myeloid Leukemia
    Gene expression
    Chromatin
    Genes
    17 Citations (Scopus)

    Epithelial-to-mesenchymal transition confers pericyte properties on cancer cells

    Shenoy, A. K., Jin, Y., Luo, H., Tang, M., Pampo, C., Shao, R., Siemann, D. W., Wu, L., Heldermon, C. D., Law, B. K., Chang, L. J. & Lu, J., Nov 1 2016, In : Journal of Clinical Investigation. 126, 11, p. 4174-4186 13 p.

    Research output: Contribution to journalArticle

    Open Access
    Pericytes
    Epithelial-Mesenchymal Transition
    Neoplasms
    Blood Vessels
    Carcinoma
    19 Citations (Scopus)

    MOF Acetylates the Histone Demethylase LSD1 to Suppress Epithelial-to-Mesenchymal Transition

    Luo, H., Shenoy, A. K., Li, X., Jin, Y., Jin, L., Cai, Q., Tang, M., Liu, Y., Chen, H., Reisman, D., Wu, L., Seto, E., Qiu, Y., Dou, Y., Casero, R. A. & Lu, J., Jun 21 2016, In : Cell Reports. 15, 12, p. 2665-2678 14 p.

    Research output: Contribution to journalArticle

    Open Access
    Histone Demethylases
    Epithelial-Mesenchymal Transition
    Tumors
    Acetylation
    Histones
    29 Citations (Scopus)

    FBXO11 promotes ubiquitination of the Snail family of transcription factors in cancer progression and epidermal development

    Jin, Y., Shenoy, A. K., Doernberg, S., Chen, H., Luo, H., Shen, H., Lin, T., Tarrash, M., Cai, Q., Hu, X., Fiske, R., Chen, T., Wu, L., Mohammed, K. A., Rottiers, V., Lee, S. S. & Lu, J., Jan 1 2015, In : Cancer Letters. 362, 1, p. 70-82 13 p.

    Research output: Contribution to journalArticle

    Ubiquitination
    Snails
    Epithelial-Mesenchymal Transition
    Neoplasms
    Ubiquitin

    Prizes

    Best Oral Presentation

    Huacheng Luo (Recipient), 2017

    Prize