• 445 Citations
  • 5 h-Index
20092019
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Personal profile

Research interests

Dr. Huacheng Luo’s current studies are focused on cancer/tumor genetics and epigenetics research fields. One of his current projects involves targeting CTCF boundary remodels chromatin domain and reprograms HOX gene transcription in acute myeloid leukemia. In this research, he employed a pooled CRISPR-Cas9 genetic knockout (KO) library screening to interrogate CTCF binding motifs in all HOX loci and identified a critical CTCF boundary (CBS7/9) located at the edge of TAD encompassing the posterior HOXA genes. He found that CBS7/9 chromatin boundary is critical for initiating and maintaining aberrant expression of posterior HOXA genes (HOXA9-HOXA13).

To further investigate the role of CBS7/9 chromatin boundary in HOX gene organization and regulation, he then carried out genome wide ChIP-seq, ATAC-seq, 4C-seq and RNA-seq analysis to examine the global changes in chromatin domain organization and corresponding gene expression patterns between control and CBS7/9 knockout AML cells. Depletion of the CBS7/9 boundary function leads to expansion of repressive chromatin structure into posterior HOXA domain, blocking enhancer/promoter chromatin accessibility and their associated regulatory networks, decreases in HOXA9 associated oncogenic transcription program and eventually prolonged survival of transplanted AML mouse models. The CTCF boundaries in the oncogene loci such as CBS7/9 may serve as novel therapeutic targets for the treatment of myeloid malignancies.

Dr. Luo is also focused on the studies of the HOX loc lncRNAs function in human normal and malignant hematopoiesis, including HOTTIP and HoxBlinc lncRNAs. He found that HOTTIP/HoxBlinc lncRNA preferentially recognizes and binds to noncoding regulatory regions in the AML genome and specifically regulates genes important for hematopoietic and myeloid lineage differentiation.

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  • 18 Similar Profiles
Chromatin Medicine & Life Sciences
Epithelial-Mesenchymal Transition Medicine & Life Sciences
Clustered Regularly Interspaced Short Palindromic Repeats Medicine & Life Sciences
Neoplasms Medicine & Life Sciences
Genes Chemical Compounds
Stem Cells Medicine & Life Sciences
Gene expression Chemical Compounds
Histone Demethylases Medicine & Life Sciences

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Research Output 2009 2019

  • 445 Citations
  • 5 h-Index
  • 6 Article
  • 3 Chapter
1 Citation (Scopus)

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Luo, H., Sobh, A., Vulpe, C. D., Brewer, E., Dovat, S., Qiu, Y. & Huang, S., Mar 31 2019, In : Journal of visualized experiments : JoVE. 145

Research output: Contribution to journalArticle

Clustered Regularly Interspaced Short Palindromic Repeats
Chromatin
Screening
Genes
Gene Expression
5 Citations (Scopus)

CTCF boundary remodels chromatin domain and drives aberrant HOX gene transcription in acute myeloid leukemia

Luo, H., Wang, F., Zha, J., Li, H., Yan, B., Du, Q., Yang, F., Sobh, A., Vulpe, C., Drusbosky, L., Cogle, C., Chepelev, I., Xu, B., Nimer, S. D., Licht, J., Qiu, Y., Chen, B., Xu, M. & Huang, S., Aug 23 2018, In : Blood. 132, 8, p. 837-848 12 p.

Research output: Contribution to journalArticle

Transcription
Acute Myeloid Leukemia
Gene expression
Chromatin
Genes
21 Citations (Scopus)

Epithelial-to-mesenchymal transition confers pericyte properties on cancer cells

Shenoy, A. K., Jin, Y., Luo, H., Tang, M., Pampo, C., Shao, R., Siemann, D. W., Wu, L., Heldermon, C. D., Law, B. K., Chang, L. J. & Lu, J., Nov 1 2016, In : Journal of Clinical Investigation. 126, 11, p. 4174-4186 13 p.

Research output: Contribution to journalArticle

Open Access
Pericytes
Epithelial-Mesenchymal Transition
Neoplasms
Blood Vessels
Carcinoma
23 Citations (Scopus)

MOF Acetylates the Histone Demethylase LSD1 to Suppress Epithelial-to-Mesenchymal Transition

Luo, H., Shenoy, A. K., Li, X., Jin, Y., Jin, L., Cai, Q., Tang, M., Liu, Y., Chen, H., Reisman, D., Wu, L., Seto, E., Qiu, Y., Dou, Y., Casero, R. A. & Lu, J., Jun 21 2016, In : Cell Reports. 15, 12, p. 2665-2678 14 p.

Research output: Contribution to journalArticle

Open Access
Histone Demethylases
Epithelial-Mesenchymal Transition
Tumors
Acetylation
Histones
30 Citations (Scopus)

FBXO11 promotes ubiquitination of the Snail family of transcription factors in cancer progression and epidermal development

Jin, Y., Shenoy, A. K., Doernberg, S., Chen, H., Luo, H., Shen, H., Lin, T., Tarrash, M., Cai, Q., Hu, X., Fiske, R., Chen, T., Wu, L., Mohammed, K. A., Rottiers, V., Lee, S. S. & Lu, J., Jan 1 2015, In : Cancer Letters. 362, 1, p. 70-82 13 p.

Research output: Contribution to journalArticle

Ubiquitination
Snails
Epithelial-Mesenchymal Transition
Neoplasms
Ubiquitin

Prizes

Best Oral Presentation

Huacheng Luo (Recipient), 2017

Prize