• 496 Citations
  • 5 h-Index
20092020

Research output per year

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Personal profile

Research interests

Dr. Huacheng Luo’s current studies are focused on cancer/tumor genetics and epigenetics research fields. One of his current projects involves targeting CTCF boundary remodels chromatin domain and reprograms HOX gene transcription in acute myeloid leukemia. In this research, he employed a pooled CRISPR-Cas9 genetic knockout (KO) library screening to interrogate CTCF binding motifs in all HOX loci and identified a critical CTCF boundary (CBS7/9) located at the edge of TAD encompassing the posterior HOXA genes. He found that CBS7/9 chromatin boundary is critical for initiating and maintaining aberrant expression of posterior HOXA genes (HOXA9-HOXA13).

To further investigate the role of CBS7/9 chromatin boundary in HOX gene organization and regulation, he then carried out genome wide ChIP-seq, ATAC-seq, 4C-seq and RNA-seq analysis to examine the global changes in chromatin domain organization and corresponding gene expression patterns between control and CBS7/9 knockout AML cells. Depletion of the CBS7/9 boundary function leads to expansion of repressive chromatin structure into posterior HOXA domain, blocking enhancer/promoter chromatin accessibility and their associated regulatory networks, decreases in HOXA9 associated oncogenic transcription program and eventually prolonged survival of transplanted AML mouse models. The CTCF boundaries in the oncogene loci such as CBS7/9 may serve as novel therapeutic targets for the treatment of myeloid malignancies.

Dr. Luo is also focused on the studies of the HOX loc lncRNAs function in human normal and malignant hematopoiesis, including HOTTIP and HoxBlinc lncRNAs. He found that HOTTIP/HoxBlinc lncRNA preferentially recognizes and binds to noncoding regulatory regions in the AML genome and specifically regulates genes important for hematopoietic and myeloid lineage differentiation.

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Research Output

  • 496 Citations
  • 5 h-Index
  • 9 Article
  • 3 Chapter

Alteration of CTCF-associated chromatin neighborhood inhibits TAL1-driven oncogenic transcription program and leukemogenesis

Li, Y., Liao, Z., Luo, H., Benyoucef, A., Kang, Y., Lai, Q., Dovat, S., Miller, B., Chepelev, I., Li, Y., Zhao, K., Brand, M. & Huang, S., Apr 6 2020, In : Nucleic acids research. 48, 6, p. 3119-3133 15 p.

Research output: Contribution to journalArticle

Open Access
  • 1 Scopus citations

    LATS kinase–mediated CTCF phosphorylation and selective loss of genomic binding

    Luo, H., Yu, Q., Liu, Y., Tang, M., Liang, M., Zhang, D., Xiao, T. S., Wu, L., Tan, M., Ruan, Y., Bungert, J. & Lu, J., Jan 1 2020, In : Science Advances. 6, 8, eaaw4651.

    Research output: Contribution to journalArticle

    Open Access
  • HOTTIP lncRNA Promotes Hematopoietic Stem Cell Self-Renewal Leading to AML-like Disease in Mice

    Luo, H., Zhu, G., Xu, J., Lai, Q., Yan, B., Guo, Y., Fung, T. K., Zeisig, B. B., Cui, Y., Zha, J., Cogle, C., Wang, F., Xu, B., Yang, F. C., Li, W., So, C. W. E., Qiu, Y., Xu, M. & Huang, S., Dec 9 2019, In : Cancer Cell. 36, 6, p. 645-659.e8

    Research output: Contribution to journalArticle

  • 5 Scopus citations

    HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

    Luo, H., Sobh, A., Vulpe, C. D., Brewer, E., Dovat, S., Qiu, Y. & Huang, S., Mar 31 2019, In : Journal of visualized experiments : JoVE. 145

    Research output: Contribution to journalArticle

  • 1 Scopus citations

    CTCF boundary remodels chromatin domain and drives aberrant HOX gene transcription in acute myeloid leukemia

    Luo, H., Wang, F., Zha, J., Li, H., Yan, B., Du, Q., Yang, F., Sobh, A., Vulpe, C., Drusbosky, L., Cogle, C., Chepelev, I., Xu, B., Nimer, S. D., Licht, J., Qiu, Y., Chen, B., Xu, M. & Huang, S., Aug 23 2018, In : Blood. 132, 8, p. 837-848 12 p.

    Research output: Contribution to journalArticle

  • 13 Scopus citations

    Prizes

    Best Oral Presentation

    Huacheng Luo (Recipient), 2017

    Prize