• 970 Citations
  • 19 h-Index
20032022
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Personal profile

Research interests

Dr. Prashant Nighot's research has been focused on intestinal mucosal homeostasis in health and disease. The lab is interested especially in the role of defective intestinal tight junction (TJ) barrier in inflammatory bowel disease (IBD), including Crohn’s Disease and ulcerative colitis. TJ barrier defects allows penetration of luminal antigens leading to excessive immune and inflammatory response.


Recent work has focused on the role of autophagy in TJ barrier regulation. Autophagy, a cell survival mechanism, degrades unnecessary, misfolded and long-lived proteins and organelles and helps cell survive under stress. The lab's pioneering studies have shown that autophagy enhances TJ barrier via degradation of pore forming TJ protein claudin-2. Currently, the lab is conducting an NIH-funded study investigating the molecular mechanisms of autophagy regulation of TJ barrier and if autophagy-mediated enhancement of TJ barrier can prevent intestinal inflammation.


Dr. Nighot’s lab is also interested in investigating targets to combat IBD. One such recent project studies the role of aryl hydrocarbon (AhR) pathway in TJ regulation. In a collaborative study, the laboratory is using a novel, non-toxic ligand to activate AhR pathway to attenuate intestinal inflammation via enhancement of TJ barrier. Other projects in the laboratory include investigating the role of matrix metalloproteinases in intestinal inflammation and an NIH-funded study of the role of chloride channel ClC-2 in mucosal TJ barrier homeostasis.


The Nighot lab's studies employ in vivo approaches including several mouse models of intestinal inflammation, in vitro cellular and molecular methods and advanced imaging techniques to examine their hypothesis. 

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  • 5 Similar Profiles
Tight Junctions Medicine & Life Sciences
Occludin Medicine & Life Sciences
Permeability Medicine & Life Sciences
Myosin-Light-Chain Kinase Medicine & Life Sciences
Sodium-Hydrogen Antiporter Medicine & Life Sciences
Autophagy Medicine & Life Sciences
Intestinal Mucosa Medicine & Life Sciences
Swine Medicine & Life Sciences

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Projects 2014 2022

Tight Junctions
Autophagy
Inflammation
Inflammatory Bowel Diseases
Tight Junction Proteins
Tight Junctions
Occludin
Tight Junction Proteins
Caveolin 1
Caveolae

Research Output 2003 2019

  • 970 Citations
  • 19 h-Index
  • 29 Article
  • 2 Review article
  • 1 Chapter
  • 1 Comment/debate

Intestinal epithelial tight junction barrier regulation by autophagy-related protein ATG6/beclin 1

Wong, M., Ganapathy, A. S., Suchanec, E., Laidler, L., Ma, T. & Nighot, P., May 1 2019, In : American Journal of Physiology - Cell Physiology. 316, 5, p. C753-C765

Research output: Contribution to journalArticle

Tight Junctions
Occludin
Autophagy
Caco-2 Cells
Endocytosis
2 Citations (Scopus)

Lipopolysaccharide-Induced Increase in Intestinal Permeability Is Mediated by TAK-1 Activation of IKK and MLCK/MYLK Gene

Nighot, M., Rawat, M., Al-Sadi, R., Castillo, E. F., Nighot, P. & Ma, T., Apr 1 2019, In : American Journal of Pathology. 189, 4, p. 797-812 16 p.

Research output: Contribution to journalArticle

Myosin-Light-Chain Kinase
Tight Junctions
Lipopolysaccharides
Permeability
Genes

Pathophysiology of avian intestinal ion transport

Nighot, M. & Nighot, P., Feb 28 2018, In : World's Poultry Science Journal. 74, 2, p. 347-360 14 p.

Research output: Contribution to journalReview article

ion transport
pathophysiology
malabsorption
intestinal absorption
poultry production
4 Citations (Scopus)

Tight Junctions and the Intestinal Barrier

Ma, T., Nighot, P. & Al-Sadi, R., Mar 28 2018, Physiology of the Gastrointestinal Tract: Sixth Edition. Elsevier Inc., Vol. 1-2. p. 587-639 53 p.

Research output: Chapter in Book/Report/Conference proceedingChapter

Tight Junctions
Gastrointestinal Tract
Epithelium
Epithelial Cells
Bacterial Antigens
9 Citations (Scopus)
Occludin
Caveolin 1
Tight Junctions
Caveolae
Caveolins