Project: Research project

Project Details


The overall objective of this proposal is to examine alterations in the
plasma lipoprotein profile, hepatic lipoprotein producton, skeletal muscle
and adipose tissue lipoprotein lipase activity, and vessel and tissue
pathology as influenced by age, exercise, and hyperlipemia. Our
experimental design allows a longitudinal assessment of these three factors
separately and together. In addition, our design is such that the effect
of exercise on lipid and lipoprotein pathophysiology can be studied
subsequent to the existance of a relatively long-term diet induced
hyperlipemia. A compositional analysis (cholesterol--esterified and
unesterified, phospholipid, triglyceride, and protein) of plasma very low
density lipoprotein (VLDL), low density lipoprotein (LDL), and high density
lipoprotein (HDL), and hepatic VLDL, LDL and HDL will be done following
isolation of the lipoprotein classes by preparative ultracentrifugation.
Skeletal muscle and adipose tissue lipoprotein lipase activity will be
measured. Total carcass, liver, atora, and adipose tissue cholesterol will
be determined. A histological examination of the aorta will be done.
Fatty infiltration of the liver, heart, and kidney will be assessed by
histological examination. These studies will be of particular importance
in advancing our understanding of the influence of age on the plasma
lipoprotein profile and hepatic lipoprotein production. Moreover, this
proposed investigation will clarify the influence of hyperlipemia and
exercise on modifying changes in lipid and lipoprotein metabolism and
pathophysiology associated with aging.
Effective start/end date1/1/901/1/90


  • National Heart, Lung, and Blood Institute


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