AUTOMATED PEPTIDE SYNTHESIS AND PURIFICATION

Project: Research project

Description

We are requesting funds to purchase equipment necessary for synthesizing
specific peptides as needed by members of the listed user group. The
equipment includes an Applied Biosystems automated solid phase peptide
synthesizer and a preparative HPLC from Sota Chromatography, Inc., for
purification of peptides generated by the Applied Biosystems synthesizer.
The primary user group represents a sizeable subset of the faculties of the
Molecular Biology and Biology Departments at Princeton and includes all or
almost all of the research groups at Princeton that we have identified as
likely to require this capability in the pursuit of their current research. The primary use of the proposed facility is to generate peptides to be used
as immunogens to elicit antibodies that recognize a specific protein, or
domain of a protein, of biological interest. This technology will be
applied in the pursuit of a number of NIH funded research projects. These
include regulation of plasmid propagation in yeast, genetic analysis of
protein export, mutator genes in E. coli, regulation of development in
Caulobacter crescentus, outer membrane structure/function analysis in E.
coli, molecular analysis of Drosophila sex determination, adenovirus gene
expression, and analysis of p53, a cellular tumor antigen. The
availability of this instrument will promote the expeditious completion of
these projects.
StatusFinished
Effective start/end date6/1/868/31/94

Funding

  • National Institutes of Health

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Peptides
Sex Determination Analysis
Research
Escherichia coli
Tumor Suppressor Protein p53
Financial Management
Adenoviridae
Drosophila
Chromatography
Proteins
Plasmids
Yeasts
High Pressure Liquid Chromatography
Technology
Equipment and Supplies
Membranes
Antibodies
Genes