BIOLOGY OF SV40-SPECIFIC TRANSPLANTATION ANTIGEN

Project: Research project

Description

The main objective of this renewal application is to define, at the
molecular level, the antigenic sites on SV40 T antigen recognized
by the cytotoxic T lymphocytes (CTL) in association with self coded
major histocompatibility complex class I or class II glycoproteins.
We have identified at least three distinct CTL recognition sites
in the amino terminal half and one site in the carboxy terminal
half of SV40 T antigen. Using CTL clones and SV40 deletion
mutants, we are now proposing to, (1) determine the identify of
amino acids in CTL recognition sites on T antigen and the influence
of amino acids within the sites on CTL recognition in association
with H-2 antigens; (2) analyses of antigenic loss variants that
have escaped CTL mediated lysis for alteration in SV40 T antigen
CTL recognition antigenic sites; (4) investigate the nature of T
antigen recognized by CTL; and (5) study the contribution of
individual antigenic sites to CTL mediated response. The approach
utilized here will allow us to relate the role of SV40 T antigen
in transformation and in inducing a CTL immune response to the
antigenic sites on SV40 T antigen in the host undergoing
oncogenesis.
StatusFinished
Effective start/end date6/1/7811/30/15

Funding

  • National Institutes of Health: $351,876.00
  • National Institutes of Health: $374,436.00
  • National Institutes of Health: $631,071.00
  • National Institutes of Health: $363,051.00
  • National Institutes of Health: $374,388.00
  • National Institutes of Health: $386,069.00

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Polyomavirus Transforming Antigens
Immunotherapy
T-Lymphocytes
Neoplasms
Viral Tumor Antigens
Histocompatibility Antigens
Adoptive Immunotherapy
Tumor Microenvironment
Adoptive Transfer
Transgenic Mice
Brain Neoplasms
Melanoma
T-Lymphocyte Epitopes
Epitopes
Radiation
Cellular Microenvironment
Neoplasm Antigens