CATECHOL-ESTROGENS, CNS FUNCTION, AND REPRODUCTION

Project: Research project

Description

The work to date has focused on establishing and validating an assay for the
enzyme(s) that convert estrogens to their catechol form ("estrogen
catecholase(s)"). The assay will be used 1) to determine the anatomical sites
in brains of rats where catechol estrogens are formed, 2) whether they are
formed in neurons or in glial cells and 3) if there are changes in the level of
activity of "estrogen catecholase(s)" with changes in reproductive status of the
animal. The influence of catechol estrogens on sexual differentiation of rat
brain is also being evaluated. Studies are also planned to use
catecholaminergic neuronal cells in tissue culture to determine whether adding
catechol estrogens to the culture medium alter various indices of
catecholaminergic function, including tyrosine hydroxylase,
catechol-O-methyltransferase, monoamine oxidase, the uptake of tyrosine
hydroxylase and of catecholamines.
StatusFinished
Effective start/end date9/1/789/30/86

Funding

  • National Institutes of Health

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Catechol Estrogens
Reproduction
Estrogens
Catechol O-Methyltransferase
Sex Differentiation
Monoamine Oxidase
Tyrosine 3-Monooxygenase
Neuroglia
Catecholamines
Culture Media
Neurons
Brain