Project: Research project

Project Details


The work to date has focused on establishing and validating an assay for the
enzyme(s) that convert estrogens to their catechol form ("estrogen
catecholase(s)"). The assay will be used 1) to determine the anatomical sites
in brains of rats where catechol estrogens are formed, 2) whether they are
formed in neurons or in glial cells and 3) if there are changes in the level of
activity of "estrogen catecholase(s)" with changes in reproductive status of the
animal. The influence of catechol estrogens on sexual differentiation of rat
brain is also being evaluated. Studies are also planned to use
catecholaminergic neuronal cells in tissue culture to determine whether adding
catechol estrogens to the culture medium alter various indices of
catecholaminergic function, including tyrosine hydroxylase,
catechol-O-methyltransferase, monoamine oxidase, the uptake of tyrosine
hydroxylase and of catecholamines.
Effective start/end date1/1/901/1/90


  • Eunice Kennedy Shriver National Institute of Child Health and Human Development

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