DEVELOPMENTAL ROLE OF (TAG 1) IN TRANSGENIC ZEBRAFISH

Project: Research project

Project Details

Description

DESCRIPTION (adapted from investigator's abstract): This proposal seeks to
investigate the in vivo role of tag I (transiently expressed axon glycoprotein)
through the generation and analysis of transgenic zebrafish (Danio rerio that
can be produced to misexpress this molecule at specific stages of development.
tag I is a cell adhesion molecule of the immunoglobulin superfamily that is
expressed transiently by a subset of embryonic cells and serves as a fertile
substrate for neurite outgrowth in vitro. Its pattern of expression in the
differentiating neurons has implicated it in the processes of axon outgrowth,
selective fasciculation, target recognition and cell migration. Although
homologs of zebrafish tag I have been cloned in the rat (TAG-1), chick (axonin
1), and human(TAX-1), the in vivo role of this moIecule has seen limited
analysis. The zebrafish represents an ideal model system to investigate the
function of tag I because it is a vertebrate embryo that is amenable to a
variety of cellular, molecular and genetic techniques. This proposal seeks to
1) construct zebrafish tag I transgenes that have been linked to green
fluorescent protein(GFP) and are driven by the zebrafish at shock promoter; 2)
create stable transgenic lines harboring these constructs; and 3) analyze the
consequences of misexpressing tag I at different stages of development. A
better understanding of the precise role tag I plays in neuronal development
could increase our understanding of how the intricate connections of the
nervous system are established, as well as suggest means for therapeutic
intervention after neuronal injury. Funding through this AREA grant will allow
unique learning experiences for undergraduates and facilitate the principal
investigator's transition to an independent investigator.
StatusFinished
Effective start/end date6/1/995/31/03

Funding

  • Eunice Kennedy Shriver National Institute of Child Health and Human Development

Fingerprint

Explore the research topics touched on by this project. These labels are generated based on the underlying awards/grants. Together they form a unique fingerprint.