Effects of ethanol on cell cycle regulation and DNA repair in neural progenitors.

Project: Research project

Description

DESCRIPTION (provided by applicant): The teratogenic effects of alcohol consumption on the developing brain can lead to devastating structural, neurologic, and psychologic impairments. The primary goal of this project is to learn how ethanol exerts its effects on brain development by exploring the relationship between ethanol exposure, DNA repair, and cell cycle regulation. Our hypothesis is that ethanol inhibits proliferation of neural stem cells through disruption of genomic replication and repair by cell cycle regulatory molecules. We will approach this issue by investigating the effects of variable ethanol exposure on the cell cycle kinetics, genomic damage, and differential gene transcription of neural stem cells (NSCs). We will also explore the role of the securin gene (PTTG1) in ethanol-induced changes in NSCs. We will study the effects of increased securin expression on NSC proliferation and DNA-repair pathways following ethanol exposure. This research will provide a greater understanding of the mechanisms by which alcohol impairs brain development and cellular division. Such knowledge has widespread applications in fetal alcohol spectrum disorder, or chronic alcoholism.
StatusFinished
Effective start/end date5/25/095/24/12

Funding

  • National Institutes of Health: $30,592.00
  • National Institutes of Health: $30,388.00
  • National Institutes of Health: $39,644.00

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DNA Repair
Cell Cycle
Ethanol
Neural Stem Cells
Securin
Brain
Fetal Alcohol Spectrum Disorders
Alcohol Drinking
Nervous System
Alcoholism
Genes
Alcohols
Cell Proliferation
Research