ELECTROANALYTICAL PROBES OF EXOCYTOSIS

Project: Research project

Project Details

Description

DESCRIPTION: (adapted from applicant's abstract) This proposal concerns the
development and application of electrochemical methods for measurements of
single exocytosis events. The overall goal of the proposed work is to
further understanding of neurotransmitter exocytosis. This is the basic
process by which neurotransmitters and hormones are released from cells to
initiate chemical communication. Yet, a great deal is still unknown about
the function of the molecular mechanism of exocytosis. The applicants
propose to develop new strategies for manufacturing smaller electrodes that
can be placed in the synapse and should theoretically be useful to make
enzyme electrodes with millisecond response time. The experiments proposed
involve 1) investigation of events that involve cellular control of the
timing of exocytosis; 2) investigation of sites of exocytosis outside the
synapse and the target receptors for this release; 3) use of a recently
developed electrochemical model of exocytosis to examine the effects of
pharmacological agents on neurotransmitter concentration and vesicle size;
4) development of methodology to examine the role of vesicle-docking
proteins on exocytotic release; 5) development of strategies to
electrochemically measure exocytosis events in single synapses and 6)
preparation of enzyme electrodes with submicron tip diameter. The
successful completion of the work proposed will further develop the
technology available for measurements of exocytotic events and will provide
a significant step toward understanding the molecular basis of exocytosis.
StatusFinished
Effective start/end date5/1/984/30/02

Funding

  • National Institute of General Medical Sciences: $143,211.00

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