ENERGY TRANSDUCTION BETWEEN MEMBRANES

Project: Research project

Description

The goal of the proposed research is to understand biological
energy transduction between membranes using the Gram-negative bacterium
E. coli as the model system. In E. coli, cytoplasmic membrane energy is
coupled to the active transport of important nutrients (iron-bearing
sideropheres and vitamin B12) across the outer membrane. The mechanism
of energy transduction remains to be elucidated. Several proteins have
been shown genetically to participate in the phenomenon, most likely in
the form of energy transduction complexes. Emphasizing TonB protein, the
protein most directly involved in energy transduction, we will attempt to
characterize the structure, function, and regulation of energy
transduction complexes. The specific aims of this project are 1.) to
determine the composition and stoichiometries of TonB-dependent energy
transduction complexes. 2.) to elucidate the role of chemical
modifications in TonB-dependent energy transduction. 3.) to delineate the
mechanism of coupling between the cytoplasmic membrane proton-motive
force and the energy-requiring processes of the outer membrane. 4.) to
characterize the mechanisms of transcriptional regulation of TonB.
Research in this area will have important ramifications for understanding
energy transduction, protein-protein interactions, and membrane phenomena
in general.
StatusFinished
Effective start/end date8/1/914/30/14

Funding

  • National Institutes of Health: $466,595.00
  • National Institutes of Health: $398,544.00
  • National Institutes of Health
  • National Institutes of Health: $323,836.00
  • National Institutes of Health
  • National Institutes of Health: $157,197.00
  • National Institutes of Health: $131,294.00
  • National Institutes of Health: $475,385.00
  • National Institutes of Health: $469,997.00
  • National Institutes of Health: $327,716.00
  • National Institutes of Health: $382,594.00
  • National Institutes of Health: $135,191.00
  • National Institutes of Health
  • National Institutes of Health: $347,501.00
  • National Institutes of Health
  • National Institutes of Health: $452,231.00
  • National Institutes of Health: $200,241.00
  • National Institutes of Health
  • National Institutes of Health: $273,396.00
  • National Institutes of Health: $399,495.00
  • National Institutes of Health: $398,302.00
  • National Institutes of Health: $73,400.00
  • National Institutes of Health

Fingerprint

Membranes
membranes
Iron
nutrients
Gram-Negative Bacteria
Cell Membrane
Proteins
Nutrients
energy
Siderophores
Periplasm
Membrane Transport Proteins
Escherichia coli
Vitamin B 12
phosphorylation
energy sources
Communicable Diseases
proteins
Machinery
Molecular Motor Proteins