EVOLUTION OF GENE REGULATION IN DROSOPHILA

Project: Research project

Description

The expression of glucose dehydrogenase (GO) is either monophasic or
biphasic in various species within the genus Drosophila. The majority of
species exhibit a single peak of GO activity (monophasic) during the pupal
stage. Four subgroups within the melanogaster species group exhibit two
peaks of GO activity: one peak during the pupal stages and one peak which
is limited to the ejaculatory duct of males. The expression of male
limited GO is correlated with a distinct ejaculatory duct morphology. GO
has been shown to be required for the process of eclosion and is
transferred from males to females during copulation in Drosophila
melanogaster (one of the biphasic species). The primary objective of the
proposed research is to elucidate the evolution of GO regulation in a few
representative species within the genus Drosophila. Two monophasic species
(D. pseudoobscura and D. takahashii) and two biphasic species (D.
melanogaster and D. eugracilis) have been chosen for investigation. Three
experimental methods will be used to examine GO regulation at various
levels of control: DNA sequence analysis, gene transformation, and
imaginal disc transplantation. Interspecific gene transformation and
imaginal disc transplantation experiments will allow us to explore the GO
regulatory differences in vivo. The combination of these two methods
offers a novel approach to investigate the evolution of regulatory
systems. In addition, the proposed research should add to our knowledge of
how tissue and developmental specific factors control gene expression.
StatusFinished
Effective start/end date12/1/843/31/99

Funding

  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $197,697.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $205,532.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health

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Glucose 1-Dehydrogenase
Drosophila
glucose
genes
Genes
Melanogaster
Ecdysterone
Gene Expression
Drosophila pseudoobscura
Cytogenetics
copulation
Fertility
eclosion
History
sequence analysis
Genome
nucleotide sequences
gene expression
research planning
gender