HEAVY ATOM CLUSTERS AS SPECIFIC LABELS

  • Bewley, Maria (PI)
  • WALL, JOSEPH (PI)
  • HAINFELD, JAMES (PI)
  • LIN, BETH (PI)
  • ANDERSON, DWIGHT (PI)
  • BARCENA, MONTSERRAT (PI)
  • BERRIOS, MIGUEL (PI)

Project: Research project

Project Details

Description

When studying a biological structure, ' it is frequently important
to identify a componentor specific site. An elegant way to achieve
this is to use labels that tag specific sites or subunits. This work
focuses on: a) Development of small, high-resolution labels that may
be visualized by electron microscopy (EK, x-ray scattering, or other
means, b) Application of these labels to a variety of biological
structures including transfer RNA (tRNA) and ribosomes, chaperonins,
blood-clotting proteins and complexes, RNAse P, chromosomes, DNA
fi-agments, multienzyme complexes, and viruses and phages, and c)
Exploring the use of these labels by EK atomic force microscopy (AFK,
scanning tunneling microscopy (STIM), electron energy loss
spectroscopy (EELS), and synchrotron x-ray anomalous scattering. Some
of these new labels may have application in medicine, e.g., for cancer
therapy, but this grant does not cover fimding for such applications.
Several gold clusters (An. , and Aul.4.)Itungstate clusters (WI I),
and a larger uranium complex have been developed winch have proven
useful in studying various biological structures by EM. They also
have been used to develop improved immunolabels. 'Me clusters can be
covalently coupled to a variety of finictional groups such as thiols,
amines, carboxyls, phosphates, or carbohydrates. This endeavor will
provide unique- high-resolution structural information about specific
functional sites that is difficult or impossible to obtain by other
means.
StatusFinished
Effective start/end date10/1/973/31/99

Funding

  • National Center for Research Resources