HUMAN AND MOUSE GENES AND DOPAMINERGIC DISORDERS

Project: Research project

Project Details

Description

The rewarding/reinforcing effects of cocaine are produced by blocking
reuptake of dopamine by the dopamine transporter (DAT). The DAT gene is
expressed in dopaminergic neurons of the ventral midbrain, and serves as
the only currently-available marker expressed almost exclusively by these
cells. During this FY, we have completed isolation of the complete human
DAT gene, and identified frequencies and patterns of linkage
disequilibrium between allelic sequence variants in the second, sixth,
and ninth exons. Identification of homologous muring genomic sequences
from 129 strains has allowed construction of homologous recombinant DAT
knockout mice for neurobiologic studies. Work to confirm or extend
initial associations of DAT gene markers with attention deficit
hyperactivity disorder and with the paranoia that can accompany cocaine
abuse continued as more subjects were characterized, while failure of
markers at the DAT or synaptic vesicular monoamine transporter (VMAT2)
locus to cosegregate with schizophremnia in several pedigrees was
reported.
StatusNot started

Funding

  • National Institute on Drug Abuse
  • National Institute on Drug Abuse
  • National Institute on Drug Abuse

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