HYDROGENATED FATTY ACID EFFECTS ON LIPID/LIPOPROT METAB

Project: Research project

Project Details

Description

This proposal addresses important questions about the effects of dietary
fatty acids on plasma lipids and measures of hemostasis that can be most
effectively answered utilizing a multicenter study design. Experiments are
proposed for a Penn State Field Center that are compatible with the
Cooperative Diet Studies Project. Our guiding hypothesis is that the long-
chain saturated fatty acids (SFA) are atherogenic (with the exception of
stearic acid) by virtue of their effects on two major risk factors for
coronary heart disease, blood lipids and thrombosis. We propose to study
young men and women and middle-aged men and women and children (families)
to gain a better understanding of the effects of age, gender, genetics and
baseline cholesterol level on the plasma cholesterol response to dietary
fatty acids and cholesterol. Utilizing a randomized, double-blinded,
cross-over study design subjects will be fed carefully formulated
experimental diets that provide 30% of calories from fat. Our proposed
experiments will address the following key questions: 1) What are the
relative hypercholesterolemic effects of the major long-chain SFA
(myristic, palmitic and stearic acid)? Is the response potentiated by
dietary cholesterol (69 mg/day vs.300 mg/day)? 2) What are the relative
cholesterolemic effects of stearic versus linoleic acid and linoleic versus
oleic acid? 3) What are the effects of trans fatty acids on plasma lipids
and Lp(a) levels? and 4) How do fatty acids affect hemostasis? To answer
these questions we propose to conduct four feeding studies. Whole food
diets that contain special fat-modified meat products will enable us to
study the interactive effects of dietary fatty acids and cholesterol. Our
primary lipid endpoints are total cholesterol, triglycerides, LDL-C and
HDL-C. Measures of hemostasis include fibrinogen, factor VII activity,
plasminogen and PAI-1. Urinary 6-keto-PGF1 alpha and TBX2 also will be
quantified to examine dietary fatty acid effects on platelet function. In
addition to the major studies described, four ancillary studies are
proposed to increase our understanding of the effects of dietary fatty
acids on lipoprotein metabolism and immune function. Our experimental
design enables us also to study dietary assessment methodologies.
StatusFinished
Effective start/end date9/30/928/31/99

Funding

  • National Heart, Lung, and Blood Institute
  • National Heart, Lung, and Blood Institute
  • National Heart, Lung, and Blood Institute
  • National Heart, Lung, and Blood Institute

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