Imaging neurovascular coupling and functional connectivity in the behaving mouse

Project: Research project

Description

DESCRIPTION (provided by applicant): The goal of this project is to quantify how natural behaviors modulate hemodynamic signals in the cortex, and to measure their coupling to neural activity. We will concurrently measure neural activity and hemodynamic signals, using electrophysiology, intrinsic optical signal imaging and two-photon laser scanning microscopy, in the cortex of awake, head-fixed mice. Mice will be free to alternate between running, grooming or quiescent behaviors on top of a spherical treadmill. We will use pharmacology to dissect the relative contributions of central neural and peripheral cardiovascular mechanisms in controlling cerebral blood flow. The neurovascular impulse response function, which defines the relationship between neural activity and blood flow, will be quantified across behaviors to test the constancy of neurovascular coupling. Lastly, we will test whether functional networks, areas with correlated blood flow during rest, are similarly correlated during locomotion. Understanding if, and how, the behavioral state modulates the cortical hemodynamic response, the coupling of blood flow to neural activity, and functional connectivity, are all critical for the interpretationof hemodynamic signals.
StatusActive
Effective start/end date3/1/131/31/24

Funding

  • National Institutes of Health: $317,598.00
  • National Institutes of Health: $317,996.00
  • National Institutes of Health: $347,422.00
  • National Institutes of Health: $318,373.00
  • National Institutes of Health: $315,547.00
  • National Institutes of Health: $316,504.00

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Hemodynamics
Cerebrovascular Circulation
Optical Imaging
Electrophysiology
Grooming
Personal Autonomy
Locomotion
Photons
Confocal Microscopy
Running
Pharmacology
Head
Neurovascular Coupling
Brain
Polarography