Interstitial Norepinephrine and Exercise

Project: Research project

Project Details

Description

DESCRIPTION (provided by applicant): The long-range goal of the principal
investigator is to examine the mechanisms that regulate the autonomic nervous
system. The specific goals of this program are to better understand the
mechanisms regulating interstitial NE concentrations ((NE)i during exercise in
humans. NE release evokes smooth muscle contractions and vasoconstriction. Our
first aim in this proposal is to examine in human subjects the effects of
changes in the interstitial concentration of potassium, nitric oxide and ATP on
(NE)i. We hypothesize that potassium, NO and ATP will raise (NE)i. We will then
explore the mechanisms by which these substances mediate their postulated
effects. Our second aim is to examine and compare (NE)i in contracting and
resting muscle during one-legged exercise. We hypothesize the (NE)i will be
greater in contracting than in non-contracting muscle. We will then attempt to
locally and selectively modify the interstitial milieu in order to better
understand the determinants of (NE)i during exercise. Preliminary data from our
laboratory suggests that the (NE)i in skeletal muscle is approximately 5.5
times the plasma concentration and is likely to be far more reflective of
events within the neurovascular junction than plasma NE determinations or
measurements of NE spillover (determined with radiotracer methods). A key
feature of this proposal is that we will use microdialysis techniques in human
subjects to determine (NE)i. To the best of our knowledge, experiments such as
these have never been performed in human subjects. The work will be performed
in a NIH funded General Clinical Research Center (GCRC) housed in the College
of Medicine at the Hershey Medical Center, Hershey, PA.
StatusFinished
Effective start/end date5/1/024/30/13

Funding

  • National Heart, Lung, and Blood Institute: $307,800.00
  • National Heart, Lung, and Blood Institute: $307,800.00
  • National Heart, Lung, and Blood Institute: $387,750.00
  • National Heart, Lung, and Blood Institute: $307,800.00
  • National Heart, Lung, and Blood Institute: $300,567.00
  • National Heart, Lung, and Blood Institute: $387,750.00
  • National Heart, Lung, and Blood Institute: $307,800.00

Fingerprint

Explore the research topics touched on by this project. These labels are generated based on the underlying awards/grants. Together they form a unique fingerprint.