MOLECULAR BIOLOGY OF FIBROBLAST PNEUMOCYTE FACTOR

Project: Research project

Description

Glucocorticoids enhance fetal mammalian lung maturation as signaled by
increased production of surfactant by the Type II pneumocytes. Our
laboratory has reported that glucocorticoid effect on surfactant production
is indirect. Glucocorticoids stimulate fetal lung fibroblasts to
synthesize a fibroblast pneumocyte factor (FPF) which in turn stimulates
surfactant production by the Type II alveolar epithelial cells. Since lung immaturity (respiratory distress syndrome - RDS) remains a major
public health problem, further understanding of this system is necessary.
Therefore, I propose to elucidate the mode of action of glucocorticoids on
FPF synthesis at the protein and RNA level and determine whether
glucocorticoid affects the production of FPF at a pretranslational or a
posttranslational level. Subsequently, the role of other hormones on the
regulation of FPF synthesis will also be examined at the protein and RNA
level. Androgens for example would be of a particular interest in order to
understand the differences in FPF synthesis and secretion between males and
females. Finally other regulatory hormones that affect the synthesis of
FPF will be further examined for their role on FPF production during
different development stages. Any significant observations will be further
substantiated by in vivo experiments.
StatusFinished
Effective start/end date7/1/856/30/87

Funding

  • National Institutes of Health
  • National Institutes of Health

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Alveolar Epithelial Cells
Molecular Biology
Fibroblasts
Glucocorticoids
Surface-Active Agents
Lung
Hormones
Androgens
Proteins
RNA
Health