MOLECULAR EPIDEMIOLOGY OF MYCOBACTERIUM AVIUM

Project: Research project

Project Details

Description

DESCRIPTION (adapted from the Abstract): In the United States, infection
with Mycobacterium avium is the most common disease of bacterial origin
among patients with the acquired immunodeficiency syndrome (AIDS). Despite
the great morbidity and mortality associated with M. avium infections, we
lack a detailed understanding of the epidemiology of this pathogen. For
instance, we do not yet know whether an animal reservoir exists of M. avium
clones that cause disease in AIDS patients; we have much to learn about the
transmission of the bacterium during the course of infection. In this
application, the Principal Investigator proposes studies that will (a)
provide a comprehensive understanding of the molecular epidemiology of M.
avium infections in humans (primarily, in AIDS patients) and animals, and
(b) enable the development of powerful new genetic markers for strain
identification. Preliminary studies have led the Investigator to formulate
three hypotheses: (i) the majority of M. avium infections in patients with
AIDS are caused by only a small subset of extant M. avium clones; (ii) an
animal reservoir exists of M. avium clones that may also be recovered from
AIDS patients; and (iii) automated polymerase chain reaction (PCR)-based DNA
fingerprinting strategies may be used for the rapid and unambiguous
identification of M. avium clones. To test these hypotheses, the
Investigator and his associates will use multilocus enzyme
electrophoresis--the method of choice for bacterial population genetic
studies--to determine allelic variation at metabolic enzyme loci in a sample
of more that 1,000 M. avium isolates recovered from humans (primarily, from
AIDS patients) and from domesticated and wild animals. All isolates in the
collection will also be characterized by IS1245 fingerprinting, a technique
that will provide a fine-structure epidemiologic analysis of M. avium from
humans and animals. Finally, because present methodology for M. avium
strain identification and fingerprinting are generally labor intensive and
time consuming, the researchers will evaluate the feasibility of used a
semi-automated PCR-based method (i.e., flourophore-enhanced
repetitive-element PCR) for M. avium strain identification.
StatusFinished
Effective start/end date7/1/966/30/02

Funding

  • National Institute of Allergy and Infectious Diseases
  • National Institute of Allergy and Infectious Diseases: $73,675.00
  • National Institute of Allergy and Infectious Diseases
  • National Institute of Allergy and Infectious Diseases
  • National Institute of Allergy and Infectious Diseases

Fingerprint

Explore the research topics touched on by this project. These labels are generated based on the underlying awards/grants. Together they form a unique fingerprint.