REGULATED EXPRESSION OF GENES IN TUMOR PROGRESSION--EPIDERMIS OF TRANSGENIC MICE

Project: Research project

Description

In order to assess the effects of specific genes on premalignant progression in vivo,we have generated a line of transgenic mice in which the tetracycline suppressible transactivator (tTA) is targeted to the epidermis with a keratin 5 promoter. Founders for a transgenic line with the reverse transactivator rTA in which transactivation is induced by tetracycline have also been made but they have not been tested for expression. The efficacy of the BK5/tTA transgenic as a transactivator has been demonstrated by crossing the K5/tTA lines with a transgenic line expressing a tetO/beta-galactosidase transgene. Both systemic and topical doxycycline can suppress expression of beta-galactosidase in the double transgenic mice. We have generated several tetO transgenics with different target genes including an oncogenic c-ras and a constiuitively active TGF-beta1 in order to regulate their expression at specific stages of carcinogenesis.
StatusNot started

Funding

  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health

Fingerprint

Doxycycline
Trans-Activators
Transgenic Mice
Epidermis
Gene Expression
Alopecia
Papilloma
Transgenes
Keratin-5
Tetracycline
Genes
beta-Galactosidase
Neoplasms
Hair
Carcinogenesis
Squamous Cell Carcinoma
Phenotype
Weaning
Hair Follicle
Carcinoma