REGULATION OF BILIPROTEIN BIOSYNTHESIS IN CYANOBACTERIA

Project: Research project

Project Details

Description

The cyanobacteria provide an excellent experimental system for studying the
control of gene expression in photoautotrophs. We have been applying the
powerful tools of molecular genetics and recombinant DNA technology to the
study of the regulation of gene expression in these organisms. We have
already cloned genes for several phycobiliproteins, including the genes for
phycocyanin and allophycocyanin from Synechococcus 7002. Studies are
proposed which would allow the isolation of the genes for all remaining
components of the phycobilisomes of this cyanobacterium. Studies to
characterize six cloned restriction fragments, which carry coding sequences
for phycobiliproteins including phycoerythrin from the
chromatically-adapting cyanobacterium Pseudanabaena 7409, are also
described. A series of promoter fusions to the lacZ gene of E. coli will
be constructed. These constructs will be used to examine phycobiliprotein
gene expression in cells subjected to different physiological stresses
including nutrient starvation, changes in light intensity, and changes in
light wave-length. Through the construction of defined mutations in
phycobilisome components, we hope to dissect the controls which co-ordinate
the synthesis and assembly of these proteins into functional
light-harvesting organelles. These studies should extend significantly our
understanding of gene regulation processes in the cyanobacteria.
StatusFinished
Effective start/end date3/1/862/28/87

Funding

  • National Institute of General Medical Sciences

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