Project: Research project

Project Details


This project is designed to explore spectrin and associated
cytoskeletal proteins in regard to the structure and function of
the developing nervous system. During the first 2 years of this
grant we have discovered two subtypes of brain spectrin (240/235)
and (240/235E) in the mammalian brain, and localized these
isoforms with immunocytochemistry and immunoelectron
microscopy. Brain spectrin (240/235) is located primarily in the
axons and presynaptic terminals of neurons. Brain spectrin
(240/235E) is found in the cell bodies, dendrites, and postsynaptic
terminals of neurons, as well as in certain glial cell types. The
ontogeny of these spectrin subtypes was explored in
immunoautoradiography experiments, and closely examined with
immunocytochemical procedures. Each spectrin subtype had a
distinct pattern of expression and distribution in the developing
nervous system. In this application we describe experiments
which are designed to give us a better understanding of brain
spectrin subtypes and associated spectrin binding proteins in
regard to the development of the mammalian nervous system.
The aims of this proposal are: (1) Determine the location and
distribution of brain spectrin subtypes in the developing mouse
brain using immunoelectron microscopy. (2) Quantitate brain
spectrin (240/235E) with quantitative immunodot assay. (3)
Define whether there are structural changes in the isoforms of
spectrin during development using immunoprecipitation and
peptide mapping techniques. (4) Examine whether there are two
ankyrin (syndein) subtypes in the mammalian brain utilizing
immunoblots and immunohistochemistry. (5) Determine the
ontogeny of ankyrin subtypes in the brain with immunoblots,
immunodots, immunohistochemistry, and immunoelectron
microscopy. (6) Examine the expression of amelin during
mammalian brain development employing the techniques outlined
in (5). The studies proposed constitute important and novel
inquiries into the functional significance of spectrin-like proteins
in the binding proteins in neural cells and tissues. This
investigation is part of an ongoing program in cellular and
molecular neurobiology which seeks to understand the process of
normal and abnormal brain development.
Effective start/end date1/1/901/1/90


  • National Institute of Neurological Disorders and Stroke

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