The ubiquitin-proteasome system in cardiac remodeling

  • Shearer, Gregory C. (PI)
  • GERDES, ANTHONY MARTIN (PI)
  • Wang, Xuejun (PI)
  • LI, FAQIAN (PI)
  • KACIMI, RACHID (PI)
  • KOST, CURTIS (PI)
  • Wang, Xuejun (PI)
  • Liang, Qi (PI)
  • SWALLOW, JOHN (PI)
  • SCHLENKER, EVELYN HEYMANN (PI)
  • O'CONNELL, TIMOTHY DANIEL (PI)
  • Liang, Qi (PI)
  • ARMSTRONG, STEPHEN (PI)
  • GERDES, ANTHONY MARTIN (PI)
  • HARRIS, WILLIAM (PI)
  • WANG, DAGUANG (PI)
  • O'CONNELL, TIMOTHY DANIEL (PI)
  • Liang, Qiangrong (PI)
  • ARMSTRONG, STEPHEN (PI)
  • Harmon, Erin (PI)
  • RABINOVITCH, ALEX (PI)
  • WRIGHT, CASEY (PI)
  • LI, FAQIAN (PI)

Project: Research project

Project Details

Description

A long term goal of this proposal is to delineate the mechanisms by which protein surplus
cardiomyopathies (PSCs) progress to congestive heart failure. PSCs are an emerging group of
cardiomyopathies. Crystallinopathy caused by the mutation of the alphaB-crystallin (CryAB) gene, often presents as desmin-related cardiomyopathy (DRC) and exemplifies PSCs. DRC is
characterized by aberrant desmin aggregation in muscle cells and this aggregation appears to play a central role in DRC pathogenesis. Notably, similar protein aggregates were also observed in human congestive heart failure (CHF) resulting from idiopathic dilated cardiomyopathy, a common heart disease. However, it remains unclear how abnormal protein aggregation affects myocyte functions. The current proposal focuses on the ubiquitin-proteasome system (UPS) mediated protein turnover, a cellular process essential to virtually all aspects of cell function. The central hypothesis is that aberrant protein aggregation characteristic of DRC impairs proteolytic function of the UPS, representing a nodal pathogenic process in PSCs. These specific aims will be pursued: (1) To test whether CryAB has an obligatory role in UPS function and to define a correlation (likely a causal relation) between aberrant protein aggregation and UPS impairment in intact mice. The underlying hypothesis is that aberrant protein aggregation instead of loss-of-function of CryAB impairs the UPS in crystallinopathic hearts. (2) To test a cause-effect link between aberrant protein aggregation and UPS impairment in cell culture. This is to test the hypothesis that formation of protein aggregates through expression of a mutant CryAB is sufficient to compromise UPS function. (3) To discover the identities of ubiquitylated proteins accumulated in crystallinopathy mouse hearts lusing proteomics. Underlying hypothesis is that accumulated ubiquitylated proteins include structural proteins and physiologically important regulatory proteins.
StatusFinished
Effective start/end date1/1/016/30/12

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