Transcriptomes and Proteomes of Plasmodium Vivax

  • Cui, Liwang, (PI)

Project: Research project

Description

? DESCRIPTION (provided by applicant): Plasmodium vivax is the second most prevalent and the most geographically widespread malaria parasite, causing enormous morbidity and mortality in affected populations. Research on P. vivax malaria has lagged significantly behind, largely due to the lack of a continuous in vitro culture system. This parasite selectively invades reticulocytes, making long-term culture difficult. P. vivax gametocytes, which are responsible for transmission, are produced before the onset of the clinical symptoms. Though the first P. vivax genome was published in 2008, systematic comparison of the transcriptomes and proteomes of the asexual erythrocytic cycle is lacking and we know little about the developmental biology of P. vivax gametocytes. This project, built on recent advancement in humanized mouse model to study the liver stages of this parasite and our improved in vitro culture techniques for synchronous blood stages, aims to determine the temporal transcriptomes and proteomes during P. vivax asexual erythrocytic development and gametocytogenesis using RNA sequencing and mass spectrometry technologies, respectively. The comprehensive transcriptomic and proteomic data generated from this study will enable systems biology approaches to address the fundamental biology of asexual and sexual development of this neglected malaria parasite. This information is also deemed critical for the discovery of novel drugs and vaccines for the interruption of P. vivax transmission.
StatusFinished
Effective start/end date3/15/162/28/19

Funding

  • National Institutes of Health: $169,726.00
  • National Institutes of Health: $216,632.00

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Plasmodium vivax
Proteome
Transcriptome
Parasites
Malaria
Vivax Malaria
RNA Sequence Analysis
Developmental Biology
Culture Techniques
Sexual Development
Systems Biology
Reticulocytes
Drug Discovery
Proteomics
Mass Spectrometry
Vaccines
Genome
Technology
Morbidity
Mortality