Project: Research project

Project Details


Cdc34 protein of Saccharomyces cerevisiae, a ubiquitin conjugating
enzyme, can undergo autoubiquitination. The reaction product is a
multiubiquitinated Cdc34 protein. Attachment of a multiubiquitin chain
to a protein is usually a signal for selective degradation of that
protein. Whether this is the case for Cdc34 protein is a subject of the
proposed research. We propose to identify the autoubiquitination sites
in Cdc34 protein and then alter these sites by in vitro mutagenesis to
generate autoubiquitination-defective mutants. We will then express these
mutants in Saccharomyces cerevisiae to determine whether
autoubiquitination serves to target Cdc34 protein for degradation and
whether such degradation is important in controlling the availability of
Cdc34 protein during the cell cycle. Finally, we will further define the
biochemical role of CDC34 by identifying and characterizing proteins that
interact with Cdc34 protein, either as participants in a multiprotein
complex or as substrates for Cdc34-dependent ubiquitination. These
studies will lead to important insights into the role of ubiquitination
in the control of cell growth.
Effective start/end date1/1/934/30/97


  • National Institute of General Medical Sciences
  • National Institute of General Medical Sciences
  • National Institute of General Medical Sciences


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