VITAMIN A TRANSPORT DURING LACTATION

Project: Research project

Project Details

Description

The goal of the proposed research is to obtain basic information on how
maternal diet and nutrition affect the composition of milk and the health
of the neonate. The proposal focuses on the delivery of vitamin A
(retinol) from mother to young during pregnancy and lactation. Specific
goals are: 1) to understand how diets that differ widely in fat and/or
protein content affect retinol transport in milk and neonatal stores of
vitamin A; 2) to understand how acute supplementation with retinyl acetate
or Beta-carotene to pregnant or nursing rats fed a basal vitamin A diet
changes milk vitamin A content and whether acute supplementation will
significantly expand the weanling's liver stores of vitamin A; 3) to
explore whether expanded liver and body stores of retinol have functional
effects on growth or the ability of the neonate to respond immunologically
to an antigen challenge, and 4) to obtain a comprehensive profile of the
levels of retinol and its transport protein retinol-binding protein (RBP),
as well as transthyretin (TTR) in serum of animals from pre-pregnancy
through lactation. We also plan to investigate possible differences in the
molecular form of the vitamin A transport complex in the adult and fetal
animal. In all studies, semisynthetic diets will be fed to control the
content of vitamin A, fat and protein, and food consumption will be
monitored. Sensitive assays will allow measurement of retinol
concentrations, retinyl ester patterns and fatty acid profiles in milk
samples collected from day 1 to near the end of lactation. Immunoassays
will be used to measure specifically the levels of RBP and TTR in serum of
dams or pups. Possible changes in the size and characteristics of the
retinol transport complex during gestational development will be explored
using gel permeation chromatography, electrophoresis, and immunoassays.
Because vitamin A is essential for reproduction, growth, and normal
differentiation of epithelia, it is essential to understand the effects of
maternal nutrition and the mechanisms that regulate the transport of this
nutrient from mother to young during gestation and lactation.
StatusFinished
Effective start/end date4/1/823/31/93

Funding

  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health

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