α-Helical nascent polypeptide chains visualized within distinct regions of the ribosomal exit tunnel

Shashi Bhushan, Marco Gartmann, Mario Halic, Jean Paul Armache, Alexander Jarasch, Thorsten Mielke, Otto Berninghausen, Daniel N. Wilson, Roland Beckmann

Research output: Contribution to journalArticle

129 Scopus citations

Abstract

As translation proceeds, the nascent polypeptide chain passes through a tunnel in the large ribosomal subunit. Although this ribosomal exit tunnel was once thought only to be a passive conduit for the growing nascent chain, accumulating evidence suggests that it may in fact play a more active role in regulating translation and initial protein folding events. Here we have determined single-particle cryo-electron microscopy reconstructions of eukaryotic 80S ribosomes containing nascent chains with high α-helical propensity located within the exit tunnel. The maps enable direct visualization of density for helices as well as allowing the sites of interaction with the tunnel wall components to be elucidated. In particular regions of the tunnel, the nascent chain adopts distinct conformations and establishes specific contacts with tunnel components, both ribosomal RNA and proteins, that have been previously implicated in nascent chain-ribosome interaction.

Original languageEnglish (US)
Pages (from-to)313-317
Number of pages5
JournalNature Structural and Molecular Biology
Volume17
Issue number3
DOIs
StatePublished - Mar 1 2010

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology

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    Bhushan, S., Gartmann, M., Halic, M., Armache, J. P., Jarasch, A., Mielke, T., Berninghausen, O., Wilson, D. N., & Beckmann, R. (2010). α-Helical nascent polypeptide chains visualized within distinct regions of the ribosomal exit tunnel. Nature Structural and Molecular Biology, 17(3), 313-317. https://doi.org/10.1038/nsmb.1756