Abstract
Homothallic strains of Saccharomyces cerevisiae can change mating type as often as every generation by replacing the allele at the MAT locus with a copy of mating type information present at one of two storage loci, HML and HMR, located on either end of chromosome III. Selection of the appropriate donor locus is dictated by a mating type-specific repressor protein, α2p: Cells containing α2p select HMR, whereas those lacking α2p select HML. As a repressor protein, α2p binds to DNA cooperatively with the transcriptional activator Mcm1p. Here we show that two α2p/Mcm1p-binding sites, DPS1 and DPS2, control donor selection. DPS1 and DPS2 are located ~30 kb from the left arm of chromosome III, well removed from HML, HMR, and MAT. Precise deletion of only DPS1 and DPS2 results in random selection of donor loci and in a cells without affecting selection in α cells. Reciprocally, deletion of only the α2p binding segments in each of these two sites results in selection of the wrong donor loci in α cells without affecting preference in a cells. These results suggest that Mcm1p, bound to these two sites in the absence of α2p, activates HML as donor. Binding of α2p blocks the ability of Mcm1p bound to DPS1 and DPS2 to activate HML, resulting in default selection of HMR as donor. DPS1 and DPS2 also regulate expression of several noncoding RNAs, although deletion of at least one of these RNA loci does not affect donor preference. This suggests that transcriptional activation, rather than transcription of a specific product, is the initiating event in activating the left arm of chromosome III for donor selection.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1899-1911 |
| Number of pages | 13 |
| Journal | Genes and Development |
| Volume | 11 |
| Issue number | 15 |
| DOIs | |
| State | Published - Aug 1 1997 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Genetics
- Developmental Biology
Cite this
}
α2p controls donor preference during mating type interconversion in yeast by inactivating a recombinational enhancer of chromosome III. / Szeto, Lisa; Fafalios, Maria K.; Zhong, Hualin; Vershon, Andrew K.; Broach, James.
In: Genes and Development, Vol. 11, No. 15, 01.08.1997, p. 1899-1911.Research output: Contribution to journal › Article
TY - JOUR
T1 - α2p controls donor preference during mating type interconversion in yeast by inactivating a recombinational enhancer of chromosome III
AU - Szeto, Lisa
AU - Fafalios, Maria K.
AU - Zhong, Hualin
AU - Vershon, Andrew K.
AU - Broach, James
PY - 1997/8/1
Y1 - 1997/8/1
N2 - Homothallic strains of Saccharomyces cerevisiae can change mating type as often as every generation by replacing the allele at the MAT locus with a copy of mating type information present at one of two storage loci, HML and HMR, located on either end of chromosome III. Selection of the appropriate donor locus is dictated by a mating type-specific repressor protein, α2p: Cells containing α2p select HMR, whereas those lacking α2p select HML. As a repressor protein, α2p binds to DNA cooperatively with the transcriptional activator Mcm1p. Here we show that two α2p/Mcm1p-binding sites, DPS1 and DPS2, control donor selection. DPS1 and DPS2 are located ~30 kb from the left arm of chromosome III, well removed from HML, HMR, and MAT. Precise deletion of only DPS1 and DPS2 results in random selection of donor loci and in a cells without affecting selection in α cells. Reciprocally, deletion of only the α2p binding segments in each of these two sites results in selection of the wrong donor loci in α cells without affecting preference in a cells. These results suggest that Mcm1p, bound to these two sites in the absence of α2p, activates HML as donor. Binding of α2p blocks the ability of Mcm1p bound to DPS1 and DPS2 to activate HML, resulting in default selection of HMR as donor. DPS1 and DPS2 also regulate expression of several noncoding RNAs, although deletion of at least one of these RNA loci does not affect donor preference. This suggests that transcriptional activation, rather than transcription of a specific product, is the initiating event in activating the left arm of chromosome III for donor selection.
AB - Homothallic strains of Saccharomyces cerevisiae can change mating type as often as every generation by replacing the allele at the MAT locus with a copy of mating type information present at one of two storage loci, HML and HMR, located on either end of chromosome III. Selection of the appropriate donor locus is dictated by a mating type-specific repressor protein, α2p: Cells containing α2p select HMR, whereas those lacking α2p select HML. As a repressor protein, α2p binds to DNA cooperatively with the transcriptional activator Mcm1p. Here we show that two α2p/Mcm1p-binding sites, DPS1 and DPS2, control donor selection. DPS1 and DPS2 are located ~30 kb from the left arm of chromosome III, well removed from HML, HMR, and MAT. Precise deletion of only DPS1 and DPS2 results in random selection of donor loci and in a cells without affecting selection in α cells. Reciprocally, deletion of only the α2p binding segments in each of these two sites results in selection of the wrong donor loci in α cells without affecting preference in a cells. These results suggest that Mcm1p, bound to these two sites in the absence of α2p, activates HML as donor. Binding of α2p blocks the ability of Mcm1p bound to DPS1 and DPS2 to activate HML, resulting in default selection of HMR as donor. DPS1 and DPS2 also regulate expression of several noncoding RNAs, although deletion of at least one of these RNA loci does not affect donor preference. This suggests that transcriptional activation, rather than transcription of a specific product, is the initiating event in activating the left arm of chromosome III for donor selection.
UR - http://www.scopus.com/inward/record.url?scp=0030612741&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030612741&partnerID=8YFLogxK
U2 - 10.1101/gad.11.15.1899
DO - 10.1101/gad.11.15.1899
M3 - Article
C2 - 9271114
AN - SCOPUS:0030612741
VL - 11
SP - 1899
EP - 1911
JO - Genes and Development
JF - Genes and Development
SN - 0890-9369
IS - 15
ER -