δ_Pulmonary host defenses. I. Analysis of protein and lipids in bronchial secretions and antibody responses after vaccination with Pseudomonas aeruginosa

H. Y. Reynolds, R. E. Thompson

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The antibody responses in serum and the lower respiratory tract of rabbits immunized with formalized P. aeruginosa culture were examined. After parenteral immunization, IgG agglutinative antibody was present in bronchial secretions, which was probably derived from the intravascular pool of IgG by diffusion into respiratory fluids. After intranasal vaccination, antibody activity was detected in both secretory IgA and the IgG fractions of bronchial secretions; it was considered possible that locally synthesized IgG antibody was elicited. Immune secretory IgA had an inhibitory effect on the growth of lag phase Pseudomonas organisms in addition to its agglutinative activity, both of which were specific for the immunizing serotype. IgG and bronchial secretions did not have this inhibitory effect. IgM and complement components were noticeably absent from bronchial secretions which would indicate that the immune milieu of normal lungs is quite different from that of serum.

Original languageEnglish (US)
Pages (from-to)358-368
Number of pages11
JournalJournal of Immunology
Volume111
Issue number2
StatePublished - Jan 1 1973

Fingerprint

Pseudomonas aeruginosa
Antibody Formation
Vaccination
Immunoglobulin G
Lipids
Lung
Secretory Immunoglobulin A
Proteins
Antibodies
Pseudomonas
Serum
Respiratory System
Immunoglobulin M
Immunization
Rabbits
Growth

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

@article{54ed24b54ae44f69855000c40fe2c433,
title = "δ_Pulmonary host defenses. I. Analysis of protein and lipids in bronchial secretions and antibody responses after vaccination with Pseudomonas aeruginosa",
abstract = "The antibody responses in serum and the lower respiratory tract of rabbits immunized with formalized P. aeruginosa culture were examined. After parenteral immunization, IgG agglutinative antibody was present in bronchial secretions, which was probably derived from the intravascular pool of IgG by diffusion into respiratory fluids. After intranasal vaccination, antibody activity was detected in both secretory IgA and the IgG fractions of bronchial secretions; it was considered possible that locally synthesized IgG antibody was elicited. Immune secretory IgA had an inhibitory effect on the growth of lag phase Pseudomonas organisms in addition to its agglutinative activity, both of which were specific for the immunizing serotype. IgG and bronchial secretions did not have this inhibitory effect. IgM and complement components were noticeably absent from bronchial secretions which would indicate that the immune milieu of normal lungs is quite different from that of serum.",
author = "Reynolds, {H. Y.} and Thompson, {R. E.}",
year = "1973",
month = "1",
day = "1",
language = "English (US)",
volume = "111",
pages = "358--368",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "2",

}

δ_Pulmonary host defenses. I. Analysis of protein and lipids in bronchial secretions and antibody responses after vaccination with Pseudomonas aeruginosa. / Reynolds, H. Y.; Thompson, R. E.

In: Journal of Immunology, Vol. 111, No. 2, 01.01.1973, p. 358-368.

Research output: Contribution to journalArticle

TY - JOUR

T1 - δ_Pulmonary host defenses. I. Analysis of protein and lipids in bronchial secretions and antibody responses after vaccination with Pseudomonas aeruginosa

AU - Reynolds, H. Y.

AU - Thompson, R. E.

PY - 1973/1/1

Y1 - 1973/1/1

N2 - The antibody responses in serum and the lower respiratory tract of rabbits immunized with formalized P. aeruginosa culture were examined. After parenteral immunization, IgG agglutinative antibody was present in bronchial secretions, which was probably derived from the intravascular pool of IgG by diffusion into respiratory fluids. After intranasal vaccination, antibody activity was detected in both secretory IgA and the IgG fractions of bronchial secretions; it was considered possible that locally synthesized IgG antibody was elicited. Immune secretory IgA had an inhibitory effect on the growth of lag phase Pseudomonas organisms in addition to its agglutinative activity, both of which were specific for the immunizing serotype. IgG and bronchial secretions did not have this inhibitory effect. IgM and complement components were noticeably absent from bronchial secretions which would indicate that the immune milieu of normal lungs is quite different from that of serum.

AB - The antibody responses in serum and the lower respiratory tract of rabbits immunized with formalized P. aeruginosa culture were examined. After parenteral immunization, IgG agglutinative antibody was present in bronchial secretions, which was probably derived from the intravascular pool of IgG by diffusion into respiratory fluids. After intranasal vaccination, antibody activity was detected in both secretory IgA and the IgG fractions of bronchial secretions; it was considered possible that locally synthesized IgG antibody was elicited. Immune secretory IgA had an inhibitory effect on the growth of lag phase Pseudomonas organisms in addition to its agglutinative activity, both of which were specific for the immunizing serotype. IgG and bronchial secretions did not have this inhibitory effect. IgM and complement components were noticeably absent from bronchial secretions which would indicate that the immune milieu of normal lungs is quite different from that of serum.

UR - http://www.scopus.com/inward/record.url?scp=0015694635&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0015694635&partnerID=8YFLogxK

M3 - Article

VL - 111

SP - 358

EP - 368

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 2

ER -