σ54-Dependent regulator DVU2956 switches Desulfovibrio vulgaris from biofilm formation to planktonic growth and regulates hydrogen sulfide production

Lei Zhu, Ting Gong, Thammajun L. Wood, Ryota Yamasaki, Thomas K. Wood

Research output: Contribution to journalArticle

Abstract

Microbiologically influenced corrosion causes $100 billion in damage per year, and biofilms formed by sulfate-reducing bacteria (SRB) are the major culprit. However, little is known about the regulation of SRB biofilm formation. Using Desulfovibrio vulgaris as a model SRB organism, we compared the transcriptomes of biofilm and planktonic cells and identified that the gene for σ54-dependent regulator DVU2956 is repressed in biofilms. Utilizing a novel promoter that is primarily transcribed in biofilms (Pdvu0304), we found production of DVU2956 inhibits biofilm formation by 70%. Corroborating this result, deleting dvu2956 increased biofilm formation, and this biofilm phenotype could be complemented. By producing proteins in biofilms from genes controlled by DVU2956 (dvu2960 and dvu2962), biofilm formation was inhibited almost completely. A second round of RNA-seq for the production of DVU2956 revealed DVU2956 influences electron transport via an Hmc complex (high-molecular-weight cytochrome c encoded by dvu0531–dvu0536) and the Fe-only hydrogenase (encoded by dvu1769, hydA and dvu1770, hydB) to control H2S production. Corroborating these results, producing DVU2956 in biofilms decreased H2S production by half, deleting dvu2956 increased H2S production by 131 ± 5%, and producing DVU2956 in the dvu2956 strain reduced H2S production. Therefore, DVU2956 maintains SRB in the planktonic state and reduces H2S formation.

Original languageEnglish (US)
Pages (from-to)3564-3576
Number of pages13
JournalEnvironmental microbiology
Volume21
Issue number10
DOIs
StatePublished - Oct 1 2019

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Desulfovibrio vulgaris
Hydrogen Sulfide
hydrogen sulfide
Biofilms
biofilm
Growth
sulfate-reducing bacteria
sulfate-reducing bacterium
Sulfates
Bacteria
Corrosion
gene
cytochrome c
corrosion
Electron Transport
Cytochromes c
Transcriptome
transcriptome
electron transfer
Genes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

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title = "σ54-Dependent regulator DVU2956 switches Desulfovibrio vulgaris from biofilm formation to planktonic growth and regulates hydrogen sulfide production",
abstract = "Microbiologically influenced corrosion causes $100 billion in damage per year, and biofilms formed by sulfate-reducing bacteria (SRB) are the major culprit. However, little is known about the regulation of SRB biofilm formation. Using Desulfovibrio vulgaris as a model SRB organism, we compared the transcriptomes of biofilm and planktonic cells and identified that the gene for σ54-dependent regulator DVU2956 is repressed in biofilms. Utilizing a novel promoter that is primarily transcribed in biofilms (Pdvu0304), we found production of DVU2956 inhibits biofilm formation by 70{\%}. Corroborating this result, deleting dvu2956 increased biofilm formation, and this biofilm phenotype could be complemented. By producing proteins in biofilms from genes controlled by DVU2956 (dvu2960 and dvu2962), biofilm formation was inhibited almost completely. A second round of RNA-seq for the production of DVU2956 revealed DVU2956 influences electron transport via an Hmc complex (high-molecular-weight cytochrome c encoded by dvu0531–dvu0536) and the Fe-only hydrogenase (encoded by dvu1769, hydA and dvu1770, hydB) to control H2S production. Corroborating these results, producing DVU2956 in biofilms decreased H2S production by half, deleting dvu2956 increased H2S production by 131 ± 5{\%}, and producing DVU2956 in the dvu2956 strain reduced H2S production. Therefore, DVU2956 maintains SRB in the planktonic state and reduces H2S formation.",
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σ54-Dependent regulator DVU2956 switches Desulfovibrio vulgaris from biofilm formation to planktonic growth and regulates hydrogen sulfide production. / Zhu, Lei; Gong, Ting; Wood, Thammajun L.; Yamasaki, Ryota; Wood, Thomas K.

In: Environmental microbiology, Vol. 21, No. 10, 01.10.2019, p. 3564-3576.

Research output: Contribution to journalArticle

TY - JOUR

T1 - σ54-Dependent regulator DVU2956 switches Desulfovibrio vulgaris from biofilm formation to planktonic growth and regulates hydrogen sulfide production

AU - Zhu, Lei

AU - Gong, Ting

AU - Wood, Thammajun L.

AU - Yamasaki, Ryota

AU - Wood, Thomas K.

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Y1 - 2019/10/1

N2 - Microbiologically influenced corrosion causes $100 billion in damage per year, and biofilms formed by sulfate-reducing bacteria (SRB) are the major culprit. However, little is known about the regulation of SRB biofilm formation. Using Desulfovibrio vulgaris as a model SRB organism, we compared the transcriptomes of biofilm and planktonic cells and identified that the gene for σ54-dependent regulator DVU2956 is repressed in biofilms. Utilizing a novel promoter that is primarily transcribed in biofilms (Pdvu0304), we found production of DVU2956 inhibits biofilm formation by 70%. Corroborating this result, deleting dvu2956 increased biofilm formation, and this biofilm phenotype could be complemented. By producing proteins in biofilms from genes controlled by DVU2956 (dvu2960 and dvu2962), biofilm formation was inhibited almost completely. A second round of RNA-seq for the production of DVU2956 revealed DVU2956 influences electron transport via an Hmc complex (high-molecular-weight cytochrome c encoded by dvu0531–dvu0536) and the Fe-only hydrogenase (encoded by dvu1769, hydA and dvu1770, hydB) to control H2S production. Corroborating these results, producing DVU2956 in biofilms decreased H2S production by half, deleting dvu2956 increased H2S production by 131 ± 5%, and producing DVU2956 in the dvu2956 strain reduced H2S production. Therefore, DVU2956 maintains SRB in the planktonic state and reduces H2S formation.

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