1,25-dihydroxyvitamin D3 selectively induces increased expression of the Na,KATPase β1 subunit in avian myelomonocytic cells without a concomitant change in Na,K-ATPase activity

Agnes Billecocq, William C. Horne, Munmun Chakraborty, Kunio Takeyasu, Robert Levenson, Roland Baron

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Treatment of avian myelomonocytic cells with 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) results in an approximately two fold increase in levels of Na,KATPase β1 subunit mRNA and protein (both total and plasma membrane-associated). The changes in β1 subunit expression occur in the absence of a detectable increase in expression of any of the three a subunit isoforms or in Na,K-ATPase activity. The selective induction of the expression of the β subunit in avian myelomonocytic cells by 1,25(OH)2D3 reveals a previously unobserved feature of the regulation of Na,K-ATPase expression, while the targeting of β subunit polypeptides to the plasma membrane in the absence of a corresponding increase in active Na,K-ATPase suggests that, in these cells, transport of the β subunit to the plasma membrane may be independent of its binding to the α subunit.

Original languageEnglish (US)
Pages (from-to)221-229
Number of pages9
JournalJournal of Cellular Physiology
Volume172
Issue number2
DOIs
StatePublished - Aug 1 1997

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Calcitriol
Cell membranes
Adenosine Triphosphatases
Cell Membrane
Protein Subunits
Protein Isoforms
Cells
Messenger RNA
Peptides
sodium-translocating ATPase
Proteins

All Science Journal Classification (ASJC) codes

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

Cite this

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title = "1,25-dihydroxyvitamin D3 selectively induces increased expression of the Na,KATPase β1 subunit in avian myelomonocytic cells without a concomitant change in Na,K-ATPase activity",
abstract = "Treatment of avian myelomonocytic cells with 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) results in an approximately two fold increase in levels of Na,KATPase β1 subunit mRNA and protein (both total and plasma membrane-associated). The changes in β1 subunit expression occur in the absence of a detectable increase in expression of any of the three a subunit isoforms or in Na,K-ATPase activity. The selective induction of the expression of the β subunit in avian myelomonocytic cells by 1,25(OH)2D3 reveals a previously unobserved feature of the regulation of Na,K-ATPase expression, while the targeting of β subunit polypeptides to the plasma membrane in the absence of a corresponding increase in active Na,K-ATPase suggests that, in these cells, transport of the β subunit to the plasma membrane may be independent of its binding to the α subunit.",
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1,25-dihydroxyvitamin D3 selectively induces increased expression of the Na,KATPase β1 subunit in avian myelomonocytic cells without a concomitant change in Na,K-ATPase activity. / Billecocq, Agnes; Horne, William C.; Chakraborty, Munmun; Takeyasu, Kunio; Levenson, Robert; Baron, Roland.

In: Journal of Cellular Physiology, Vol. 172, No. 2, 01.08.1997, p. 221-229.

Research output: Contribution to journalArticle

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AU - Levenson, Robert

AU - Baron, Roland

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