Treatment of avian myelomonocytic cells with 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) results in an approximately two fold increase in levels of Na,KATPase β1 subunit mRNA and protein (both total and plasma membrane-associated). The changes in β1 subunit expression occur in the absence of a detectable increase in expression of any of the three a subunit isoforms or in Na,K-ATPase activity. The selective induction of the expression of the β subunit in avian myelomonocytic cells by 1,25(OH)2D3 reveals a previously unobserved feature of the regulation of Na,K-ATPase expression, while the targeting of β subunit polypeptides to the plasma membrane in the absence of a corresponding increase in active Na,K-ATPase suggests that, in these cells, transport of the β subunit to the plasma membrane may be independent of its binding to the α subunit.
|Original language||English (US)|
|Number of pages||9|
|Journal||Journal of Cellular Physiology|
|State||Published - Aug 1997|
All Science Journal Classification (ASJC) codes
- Clinical Biochemistry
- Cell Biology