20-hydroxyecdysone mediates non-canonical regulation of mosquito vitellogenins through alternative splicing

K. N. Provost-Javier, Jason Laurence Rasgon

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Vitellogenesis is one of the most well-studied physiological processes in mosquitoes. Expression of mosquito vitellogenin genes is classically described as being restricted to female adult reproduction. We report premature vitellogenin transcript expression in three vector mosquitoes: Culex tarsalis, Aedes aegypti and Anopheles gambiae. Vitellogenins expressed during non-reproductive stages are alternatively spliced to retain their first intron and encode premature termination codons. We show that intron retention results in transcript degradation by translation-dependent nonsense-mediated mRNA decay. This is probably an example of regulated unproductive splicing and translation (RUST), a mechanism known to regulate gene expression in numerous organisms but which has never been described in mosquitoes. We demonstrate that the hormone 20-hydroxyecdysone (20E) is responsible for regulating post-transcriptional splicing of vitellogenin. After exposure of previtellogenic fat bodies to 20E, vitellogenin expression switches from a non-productive intron-retaining transcript to a spliced protein-coding transcript. This effect is independent of factors classically known to influence transcription, such as juvenile hormone-mediated competence and amino acid signalling through the target of rapamycin pathway. Non-canonical regulation of vitellogenesis through RUST is a novel role for the multifunctional hormone 20E, and may have important implications for general patterns of gene regulation in mosquitoes.

Original languageEnglish (US)
Pages (from-to)407-416
Number of pages10
JournalInsect Molecular Biology
Volume23
Issue number4
DOIs
StatePublished - Jan 1 2014

Fingerprint

Ecdysterone
Vitellogenins
ecdysterone
alternative splicing
vitellogenin
Alternative Splicing
Culicidae
Introns
Vitellogenesis
translation (genetics)
introns
vitellogenesis
Nonsense Mediated mRNA Decay
hormones
Hormones
Physiological Phenomena
Culex tarsalis
Anopheles gambiae
Juvenile Hormones
Culex

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Insect Science

Cite this

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20-hydroxyecdysone mediates non-canonical regulation of mosquito vitellogenins through alternative splicing. / Provost-Javier, K. N.; Rasgon, Jason Laurence.

In: Insect Molecular Biology, Vol. 23, No. 4, 01.01.2014, p. 407-416.

Research output: Contribution to journalArticle

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