25-Hydroxyvitamin D: Analysis and clinical application

Zengliu Su, Satya Nandana Narla, Yusheng Zhu

Research output: Contribution to journalReview article

23 Citations (Scopus)

Abstract

25-Hydroxyvitamin D (25-OHD) is one of the most popular tests requested by clinicians nowadays because in addition to bone diseases, many non-skeletal disorders have been suggested to be linked to vitamin D deficiency or insufficiency. Methodologies used in clinical laboratories include competitive vitamin D protein binding assays (CPBA), immunoassays, high performance liquid chromatography (HPLC), and liquid chromatography-tandem mass spectrometry (LC-MS/MS). In this review article, we introduce the basic metabolism and physiology of vitamin D, key issues in the methods for 25-OHD measurement currently used in most clinical laboratories, and clinical applications of 25-OHD testing. We conclude that although the methodologies for 25-OHD testing have improved significantly, considerable bias between different methods and laboratories still exists. Therefore, standardization of the method is critical. The optimal 25-OHD levels should be determined based on the standardized method. Also, more studies are needed to further determine the relationship between vitamin D deficiency or insufficiency and non-skeletal diseases as well as daily vitamin D dose requirement for reducing the risk of non-skeletal diseases.

Original languageEnglish (US)
Pages (from-to)200-205
Number of pages6
JournalClinica Chimica Acta
Volume433
DOIs
StatePublished - Jun 10 2014

Fingerprint

Vitamin D
Clinical laboratories
Vitamin D Deficiency
Vitamin D-Binding Protein
Bone Diseases
Liquid chromatography
Physiology
High performance liquid chromatography
Testing
Tandem Mass Spectrometry
Immunoassay
Metabolism
Liquid Chromatography
Standardization
Mass spectrometry
Assays
Bone
High Pressure Liquid Chromatography
25-hydroxyvitamin D

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Su, Zengliu ; Narla, Satya Nandana ; Zhu, Yusheng. / 25-Hydroxyvitamin D : Analysis and clinical application. In: Clinica Chimica Acta. 2014 ; Vol. 433. pp. 200-205.
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25-Hydroxyvitamin D : Analysis and clinical application. / Su, Zengliu; Narla, Satya Nandana; Zhu, Yusheng.

In: Clinica Chimica Acta, Vol. 433, 10.06.2014, p. 200-205.

Research output: Contribution to journalReview article

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T2 - Analysis and clinical application

AU - Su, Zengliu

AU - Narla, Satya Nandana

AU - Zhu, Yusheng

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Y1 - 2014/6/10

N2 - 25-Hydroxyvitamin D (25-OHD) is one of the most popular tests requested by clinicians nowadays because in addition to bone diseases, many non-skeletal disorders have been suggested to be linked to vitamin D deficiency or insufficiency. Methodologies used in clinical laboratories include competitive vitamin D protein binding assays (CPBA), immunoassays, high performance liquid chromatography (HPLC), and liquid chromatography-tandem mass spectrometry (LC-MS/MS). In this review article, we introduce the basic metabolism and physiology of vitamin D, key issues in the methods for 25-OHD measurement currently used in most clinical laboratories, and clinical applications of 25-OHD testing. We conclude that although the methodologies for 25-OHD testing have improved significantly, considerable bias between different methods and laboratories still exists. Therefore, standardization of the method is critical. The optimal 25-OHD levels should be determined based on the standardized method. Also, more studies are needed to further determine the relationship between vitamin D deficiency or insufficiency and non-skeletal diseases as well as daily vitamin D dose requirement for reducing the risk of non-skeletal diseases.

AB - 25-Hydroxyvitamin D (25-OHD) is one of the most popular tests requested by clinicians nowadays because in addition to bone diseases, many non-skeletal disorders have been suggested to be linked to vitamin D deficiency or insufficiency. Methodologies used in clinical laboratories include competitive vitamin D protein binding assays (CPBA), immunoassays, high performance liquid chromatography (HPLC), and liquid chromatography-tandem mass spectrometry (LC-MS/MS). In this review article, we introduce the basic metabolism and physiology of vitamin D, key issues in the methods for 25-OHD measurement currently used in most clinical laboratories, and clinical applications of 25-OHD testing. We conclude that although the methodologies for 25-OHD testing have improved significantly, considerable bias between different methods and laboratories still exists. Therefore, standardization of the method is critical. The optimal 25-OHD levels should be determined based on the standardized method. Also, more studies are needed to further determine the relationship between vitamin D deficiency or insufficiency and non-skeletal diseases as well as daily vitamin D dose requirement for reducing the risk of non-skeletal diseases.

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