3D microfilter device for viable circulating tumor cell (CTC) enrichment from blood

Siyang Zheng, Henry K. Lin, Bo Lu, Anthony Williams, Ram Datar, Richard J. Cote, Yu Chong Tai

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297 Scopus citations

Abstract

Detection of circulating tumor cells has emerged as a promising minimally invasive diagnostic and prognostic tool for patients with metastatic cancers. We report a novel three dimensional microfilter device that can enrich viable circulating tumor cells from blood. This device consists of two layers of parylene membrane with pores and gap precisely defined with photolithography. The positions of the pores are shifted between the top and bottom membranes. The bottom membrane supports captured cells and minimize the stress concentration on cell membrane and sustain cell viability during filtration. Viable cell capture on device was investigated with scanning electron microscopy, confocal microscopy, and immunofluorescent staining using model systems of cultured tumor cells spiked in blood or saline. The paper presents and validates this new 3D microfiltration concept for circulation tumor cell enrichment application. The device provides a highly valuable tool for assessing and characterizing viable enriched circulating tumor cells in both research and clinical settings.

Original languageEnglish (US)
Pages (from-to)203-213
Number of pages11
JournalBiomedical Microdevices
Volume13
Issue number1
DOIs
Publication statusPublished - Feb 1 2011

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All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Molecular Biology

Cite this

Zheng, S., Lin, H. K., Lu, B., Williams, A., Datar, R., Cote, R. J., & Tai, Y. C. (2011). 3D microfilter device for viable circulating tumor cell (CTC) enrichment from blood. Biomedical Microdevices, 13(1), 203-213. https://doi.org/10.1007/s10544-010-9485-3