4-Aminopyridine attenuates muscle atrophy after sciatic nerve crush injury in mice

Li Yue, M. A.Hassan Talukder, Anagha Gurjar, Jung Il Lee, Mark Noble, Robert T. Dirksen, Joe Chakkalakal, John Elfar

Research output: Contribution to journalArticle

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Abstract

Introduction: We recently demonstrated the beneficial effects of 4-aminopyridine (4-AP), a potassium channel blocker, in enhancing remyelination and recovery of nerve conduction velocity and motor function after sciatic nerve crush injury in mice. Although muscle atrophy occurs very rapidly after nerve injury, the effect of 4-AP on muscle atrophy and intrinsic muscle contractile function is largely unknown. Methods: Mice were assigned to sciatic nerve crush injury and no-injury groups and were followed for 3, 7, and 14 days with/without 4-AP or saline treatment. Morphological, functional, and transcriptional properties of skeletal muscle were assessed. Results: In addition to improving in vivo function, 4-AP significantly reduced muscle atrophy with increased muscle fiber diameter and contractile force. Reduced muscle atrophy was associated with attenuated expression of atrophy-related genes and increased expression of proliferating stem cells. Discussion: These findings provide new insights into the potential therapeutic benefits of 4-AP against nerve injury-induced muscle atrophy and dysfunction. Muscle Nerve 60: 192–201, 2019.

Original languageEnglish (US)
Pages (from-to)192-201
Number of pages10
JournalMuscle and Nerve
Volume60
Issue number2
DOIs
StatePublished - Aug 1 2019

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Nerve Crush
4-Aminopyridine
Muscular Atrophy
Sciatic Nerve
Muscles
Wounds and Injuries
Potassium Channel Blockers
Neural Conduction
Atrophy
Skeletal Muscle
Stem Cells
Crush Injuries
Gene Expression

All Science Journal Classification (ASJC) codes

  • Physiology
  • Clinical Neurology
  • Cellular and Molecular Neuroscience
  • Physiology (medical)

Cite this

Yue, L., Talukder, M. A. H., Gurjar, A., Lee, J. I., Noble, M., Dirksen, R. T., ... Elfar, J. (2019). 4-Aminopyridine attenuates muscle atrophy after sciatic nerve crush injury in mice. Muscle and Nerve, 60(2), 192-201. https://doi.org/10.1002/mus.26516
Yue, Li ; Talukder, M. A.Hassan ; Gurjar, Anagha ; Lee, Jung Il ; Noble, Mark ; Dirksen, Robert T. ; Chakkalakal, Joe ; Elfar, John. / 4-Aminopyridine attenuates muscle atrophy after sciatic nerve crush injury in mice. In: Muscle and Nerve. 2019 ; Vol. 60, No. 2. pp. 192-201.
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Yue, L, Talukder, MAH, Gurjar, A, Lee, JI, Noble, M, Dirksen, RT, Chakkalakal, J & Elfar, J 2019, '4-Aminopyridine attenuates muscle atrophy after sciatic nerve crush injury in mice', Muscle and Nerve, vol. 60, no. 2, pp. 192-201. https://doi.org/10.1002/mus.26516

4-Aminopyridine attenuates muscle atrophy after sciatic nerve crush injury in mice. / Yue, Li; Talukder, M. A.Hassan; Gurjar, Anagha; Lee, Jung Il; Noble, Mark; Dirksen, Robert T.; Chakkalakal, Joe; Elfar, John.

In: Muscle and Nerve, Vol. 60, No. 2, 01.08.2019, p. 192-201.

Research output: Contribution to journalArticle

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AU - Elfar, John

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Yue L, Talukder MAH, Gurjar A, Lee JI, Noble M, Dirksen RT et al. 4-Aminopyridine attenuates muscle atrophy after sciatic nerve crush injury in mice. Muscle and Nerve. 2019 Aug 1;60(2):192-201. https://doi.org/10.1002/mus.26516