[78] DNA Transformation

Ronald D. Porter

Research output: Contribution to journalArticle

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Abstract

This chapter focuses on DNA Transformation. The full power of genetics in research with any organism involves a need to be able to manipulate the genetic information by introducing new or altered DNA nucleotide sequences into a viable cell. A conjugation system involving triparental matings with Escherichia coli is now available for some cyanobacteria. The most common gene transfer mode used with these organisms, however, is DNA-mediated transformation. To date, DNA-mediated transformation has been clearly demonstrated only in those unicellular cyanobacteria that have been classified as Synechococcus or Synechocystis. A primary key to the functioning of a transformation system in any microbe is the development of cellular competence for DNA uptake. Depending on how it is developed, competence is either physiological or artificial in nature. Physiological competence describes the situation when at least some of the cells in the population possess a natural ability to productively internalize exogenous DNA without any special treatment to the cells before their exposure to the donor DNA. Artificial competence, on the other hand, is when DNA internalization by cells occurs as a result of a treatment regimen that would not be part of a normal growth cycle.

Original languageEnglish (US)
Pages (from-to)703-712
Number of pages10
JournalMethods in Enzymology
Volume167
Issue numberC
DOIs
Publication statusPublished - Jan 1 1988

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All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

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